血清miRNA-30c在胃癌早期诊断中的作用

The role of serum miRNA-30c in early diagnosis of gastric cancer

目的研究miRNA-30c（miR-30c）在胃癌早期诊断中的作用。方法收集内蒙古医科大学附属人民医院2014年8月至2017年8月胃癌患者及健康对照者的血清，实时定量聚合酶链反应检测80例中晚期胃癌、35例早期胃癌、35名健康对照者血清中miR-30c的表达量，应用受试者工作特征（ROC）曲线下面积（AUC）分析miR-30c在中晚期胃癌、早期胃癌与健康对照者中的诊断效力。结果miR-30c在中晚期胃癌组的表达量（0.45±0.11）低于早期胃癌组（0.54±0.15）（t=5.2，P〈0.05），而早期胃癌组的表达量低于健康对照组（0.61±0.12）（t=6.7，P〈0.05）。与传统肿瘤标志物相比，早期胃癌组与中晚期胃癌组miR-30c的AUC（0.92±0.03）最大，在胃癌诊断中的灵敏度与特异度分别为90%和84%；早期胃癌组与健康体检组miR-30c的AUC为0.87±0.04，在胃癌诊断中的灵敏度与特异度分别为70%和86%。结论miR-30c可作为胃癌早期血清诊断的潜在标志物。

ObjectiveTo test the value of miRNA-30c （miR-30c） in early diagnosis of gastric cancer.MethodsSerum miR-30c expression levels were detected by using quantitative real-time polymerase chain reaction in 80 patients with advanced gastric cancer, 35 patients with early stage gastric cancer and 35 healthy controls in the Affiliated People＇s Hospital of Inner Mongolia Medical University from August 2014 to August 2017. The receiver operating characteristic （ROC） curves and the area under the curve （AUC） were analyzed to test the efficacy of the miR-30c in distinguishing advanced gastric cancer, early stage gastric cancer and healthy controls.ResultsThe expression level of serum miR-30c in advanced gastric cancer （0.45±0.11） was lower than that in early stage gastric cancer （0.54±0.15） （t = 5.2, P 〈 0.05）. Compared with the healthy controls （0.61±0.12）, miR-30c was down-expressed in the serum of early stage gastric cancer patients （t = 6.7, P 〈 0.05）. Compared with the traditional tumor markers, the AUC of miR-30c was the biggest （0.92±0.03） in early stage and advanced gastric cancer groups, and the sensitivity and specificity in the diagnosis of gastric cancer were 90% and 84%, respectively. The AUC of miR-30c was 0.87±0.04 in early stage gastric cancer and healthy controls, and the sensitivity and specificity in the diagnosis of gastric cancer were 70% and 86%, respectively.ConclusionmiR-30c might be used as a potential serum biomarker for the early diagnosis of gastric cancer.