基于网络药理学研究复方钩藤降压片治疗高血压的作用机制

Explore mechanism of Compound Uncaria Hypotensive Tablet for hypertension based on network pharmacology

该文通过网络药理学研究预测复方钩藤降压片潜在的主要活性成分和作用靶点,探讨其多成分-多靶点-多通路的治疗高血压的可能作用机制。采用整合药理学平台（TCMIP）构建复方钩藤降压片成分靶标-疾病靶标网络,通过网络分析方法筛选关键节点,并在此基础上对关键节点进行通路富集分析,探索复方钩藤降压片治疗高血压可能参与的生物过程。靶点网络特征分析显示,所预测复方钩藤降压片中有35个活性成分与前列腺素内源性过氧化物合酶（PTGS1,PTGS2）、ATP合成酶（ATP1A1,ATP5A1,ATP5C1,ATP5B）等29个主要蛋白有很强的相互作用,富集到血压调节、G-蛋白耦合受体激活、心肌细胞肾上腺素能信号转导和血小板激活等15条通路,参与对高血压病理过程不同环节的调控。该研究初步揭示了复方钩藤降压片治疗高血压的潜在活性成分及其可能的作用机制,为进一步的药效物质基础和作用机制实验研究提供了理论依据。

This paper aimed to predict the active ingredients and action targets of Compound Uncaria Hypotensive Tablet for hypertension based on network pharmacology,and discuss its possible ＂ multi-components,multi-targets,and multi-pathways＂ mechanism for treatment of hypertension. The integrative pharmacological platform of traditional Chinese medicine（ TCM-IP） was used to construct the component target-disease target network of Compound Uncaria Hypotensive Tablet,and the internet analysis method was used to screen the key nodes,on which the pathway enrichment analysis was carried out to explore its possible biological process in the treatment of hypertension. Target network analysis showed that,35 predicted active ingredients of Compound Uncaria Hypotensive Tablet had a strong interaction with the prostaglandin endogenous peroxidase synthase（ PTGS1,PTGS2）,ATP synthetase（ ATP1 A1,ATP5 A1,ATP5 C1,ATP5 B） and other 29 major proteins. Network enriched analysis showed that Compound Uncaria Hypotensive Tablet participated in the regulation of hypertension in different processes of pathology,through 15 pathways such as regulating blood pressure,G protein coupled receptor activation,adrenergic myocardial cell signal transduction and platelet activation. This study revealed the potential active compounds and possible mechanism of Compound Uncaria Hypotensive Tablet for treatment of hypertension,providing theoretical references for further systematic laboratory experiments on effective compounds and action mechanism of Compound Uncaria Hypotensive Tablet.