长链非编码RNA ANRIL对膀胱尿路上皮癌细胞侵袭能力的作用

The effect of long noncoding RNA ANRIL on the invasiveness of bladder urothelial carcinoma cells

目的检测膀胱尿路上皮癌(bladder urothelial carcinoma,BUC)中长链非编码RNA Antisense Noncoding RNA在INK4 Locus(ANRIL)基因的表达,研究ANRIL基因对BUC细胞侵袭能力的调控作用。方法实时定量PCR法检测84例BUC组织和T24细胞系中ANRIL基因的表达。统计分析ANRIL基因在BUC组织的表达与BUC临床病理因素的相关性。ANRIL抑制剂(ss-ANRIL)沉默T24细胞中ANRIL的表达,Transwell小室法检测T24细胞的侵袭能力。结果与癌旁正常组织相比,ANRIL基因在BUC组织的表达显著上调。ANRIL基因在T24细胞系中的表达显著高于其在人膀胱上皮永生化细胞系SV-HUC-1中的表达。而且,ANRIL基因在高病理分级、侵袭肌层以及淋巴结转移阳性的BUC组织中的表达更高。转染ss-ANRIL能够显著抑制T24细胞中ANRIL的表达,而ANRIL沉默能够显著抑制T24细胞的侵袭能力。结论长链非编码RNA ANRIL在BUC组织及细胞系中高表达,且于BUC的侵袭转移相关,其表达沉默能够抑制BUC细胞的侵袭能力。

Objective To detect the expression of long non-coding RN A（lncRNA） antisense noncoding RNA in INK4 Locus（ANRIL） gene in bladder urothelial carcinoma（BUC） and study the effect of ANRIL gene on the invasiveness of BUC cells. Methods The expression of ANRIL gene in 84 BUC tissues and T24 cell lines was detected by real-time quantitative PCR. The correlation between the expression of ANRIL gene in BUC tissue and the clinicopathological factors of BUC was statistically analyzed. ANRIL inhibitor（ss-ANRIL） was used to silence the expression of ANRIL in T24 cells. The invasiveness of T24 cell was deteced by Transwell compartment method. Results The expression level of ANRIL gene in BUC tissue was significantly up-regulated compared with the paraancerous normal tissues. The expression level of ANRIL gene in T24 cell lines was significantly higher than that in human bladder epithelial immortalized cell line SV-HUC-1.Furthermore, the expression level of ANRIL gene was higher in BUC tissues with high pathological grade, invasion of myometrium and lymph node metastasis. The transfection of ss-ANRIL could significantly inhibit the expression of ARIL in T24 cells, while ANRIL silencing could significantly inhibit the invasiveness of T24 cells. Conclusion The overexpressed LncRNA ANRIL in BUC tissue and cell lines was related to the invasion and metastasis of BUC.