基因沉默ILK对胰腺癌细胞增殖能力的影响

The influence of silencing ILK gene on the proliferation of pancreatic cancer cells

目的研究基因沉默ILK对胰腺癌细胞(Panc-1)增殖能力的影响。方法对胰腺癌Panc-1细胞进行细胞培养,成功构建ILK-specific shRNA慢病毒载体后对Panc-1细胞进行转染,荧光显微镜下观察评估转染效率,然后通过real time PCR及Western blot方法验证干扰基因片段的有效性,应用MTT方法比较转染前后各组胰腺癌细胞增殖能力变化。结果荧光显微镜下观察可见慢病毒转染Panc-1细胞的感染效率达80%以上,转染ILK-specific shRNA慢病毒载体后,ILK mRNA及蛋白表达水平明显降低,在转染后48h、72h、96h时,细胞增殖受到了明显的抑制,且抑制趋势随时间逐渐增强(P<0.01)。结论基因沉默ILK的胰腺癌细胞增殖能力受到明显的抑制。

Objective To investigate the effect of silencing ILK gene on the proliferation of pancreatic cancer cells（Panc-1）. Methods ILK-specific shRNA lentiviral vector was transfected into the human pancreatic cancer cells（Panc-1）, and transfection efficiency was evaluated by fluorescence microscope. The validity of interference fragmentreal was verified by real time PCR and Western blot methods. The proliferation ability of pancreatic cancer cells was observed by MTT method.Results The transfection efficiency of Panc-1 cells was more than 80% under fluorescence microscope, the expression level of ILK mRNA and protein in Panc-1 cells was lower after transfection. The proliferation ability of Panc-1 cells was significantly inhibited at 48 h, 72 h, 96h after transfection, increased with time dependent manner（P 〈0.01）. Conclusion The proliferation of pancreatic cancer cells（Panc-1） was inhibited by silencing ILK gene.