miR-98通过抑制RHOA抑制结肠癌EMT

MiR-98 inhibits the EMT of colon cancer cells via suppress RHOA

目的探讨miR-98是如何通过抑制RHOA的表达结肠癌细胞上皮细胞间充质转化(EMT)的。方法采用逆转录聚合酶链反应(real time PCR)分析40对结肠癌标本中miR-98和介导EMT发生的转录因子RHOA蛋白的相关性,MIRDB预测miR-98与RHOA的结合位点后,利用荧光素酶报告基因实验检测miR-98对RHOA的靶向作用。接下来通过transwell实验验证miR-98对HCT116细胞的迁移影响,并通过western blot实验检测miR-98对RHOA、e-cad、vim蛋白的调节作用。结果结肠癌患者中miR-98和RHOA的表达水平呈负相关,miR-98与RHOA的3'UTR区域具有结合位点,miR-98可以直接作用于RHOA,抑制HCT116细胞的迁移能力,对HCT116细胞EMT的抑制在一定程度上是通过miR-98对RHOA、e-cad、vim蛋白的调节作用实现的。结论 miR-98抑制RHOA的活性,进而抑制结肠癌细胞的EMT。

Objective To investigate the effect of miR-98 on epithelial-mesenchymal transition（EMT） of human colon cancer cells. Methods The correlation between miR-98 and RHOA protein was analyzed in 40 specimens with colon cancer by real time PCR. The effect of miR-98 on HCT116 cell migration was analyzed by Transwell assay. Western blot was used to detect the effect of miR-98 on the expressions of RHOA, e-cad and vim proteins. Results MiR-98 was negatively correlated with the expression of RHOA in patients with colon cancer. miR-646 can directly act on EGFR. miR-98 inhibited the migration ability of HCT116 cells, inhibited the migration ability of HCT116 cells to some extent by miR-98 inhibition of RHOA, e-cad and vim proteins. Conclusion MiR-98 can inhibit the EMT of colon cancer cells by inhibiting the activity of RHOA.