长江三角洲白山羊优质笔料毛关键基因CMTM3启动子区甲基化分析

Methylation characteristics of CMTM3 gene promoters in high-quality brush hair in the Yangtze river delta white goat

为进一步明确趋化素样因子3(CMTM3)基因在优质笔料毛性状形成过程中的调控作用,以长江三角洲白山羊为研究对象,采用亚硫酸氢盐处理后测序法,研究关键基因CMTM3在优质笔料毛羊群体中的表达水平,分析CMTM3基因甲基化状况与优质笔料毛性状之间的相关性。结果表明:CMTM3基因在优质笔料毛羊群体中的表达量极显著低于非优质笔料毛羊群体,而优质笔料毛羊群体的甲基化水平则高于非优质笔料毛羊群体,优质笔料毛羊群体的甲基化水平为84.7%~87.4%,非优质笔料毛羊群体为80.0%~81.6%;CMTM3基因的甲基化水平与其表达量呈负相关,相关性达显著或极显著水平。这一研究提示CMTM3基因参与了优质笔料毛性状的形成过程。

To further study the role of CMTM3 gene in the formation of high quality brush hair traits,in this study,BSP was used to study the expression level of CMTM3 gene and the relationship between methylation of CMTM3 and such the formation of high quality brush hair traits in the Yangtze river delta white goat.The results showed that the relative expression level of CMTM3 gene was significantly higher in non-high quality brush hair than in high quality brush hair,and the methylation level of CMTM3 was significant higher in high quality brush hair than in non-high quality brush hair.The methylation level was 84.7%-87.4%in high quality brush hair,and the methylation level was 80.0%-81.6%in non-high quality brush hair.And the methylation level of CMTM3 was negatively correlated with the gene expression level.These results suggest that CMTM3 gene may be involved in the formation of high quality brush hair traits.