远志和蜜远志对小鼠胃肠的急性毒性作用

The Effect of Radix Polygala and Honey Stir-baking Radix Polygala on Acute Gastrointestinal Toxicity of Mice

目的考察比较中药远志和蜜远志的胃肠急性毒性。方法以灌胃给远志和蜜远志后小鼠急性毒性试验的半数致死量(LD50)和胃肠病理组织检查结果为指标进行观察。结果远志、蜜远志对小鼠口服给药的半数致死量分别为19.3 g生药/kg和31.8 g生药/kg。死亡小鼠小肠上段有水肿、瘀血。病理学检查发现:远志可损伤小鼠胃壁细胞结构,蜜远志未发现对胃壁细胞结构有明显损伤,但两者均可损伤小肠结构,引发胃、小肠黏膜下及肌层炎细胞浸润,血管充血和内皮细胞肿胀。结论远志和蜜远志均有一定胃肠毒性作用,但是蜜远志毒性相对较小。

Objective To compare the acute gastrointestinal toxicity of Radix Polygala and honey stir-baking Radix Polygala. Methods Half lethal dose（LD50） and the gastrointestinal histopathologic examination were used to evaluate the two kinds of Radix Polygala in mice after intragastric administration during acute toxicity experiments. Results The oral LD50 of Radix Polygala and honey stir-baking Radix Polygala in mice were respectively 19.3 g crude drug/kg and 31.8 g crude drug/kg. There were some edema and ecchymosis in the upper segments of small intestine in dead mice. The histopathologic results showed that Radix Polygala could induce the change of gastric parietal cell structure, while honey stir-baking Radix Polygala had not significant influence on it. The two kinds of Radix Polygala could induce small intestine injury, cause inflammatory infiltration in submucosa and muscularis of stomach and small intestine, blood vessel dilatation and endothelial swelling. Conclusion Radix Polygala and honey stir-baking Radix Polygala both showed some damage on stomach and small intestine, while the latter＇s gastrointestinal toxicity was lower than that of the former.