桔梗皂苷D的大鼠血浆药代动力学研究

Pharmacokinetic research of kikyosaponin D in rat plasma

目的:研究桔梗皂苷D在大鼠体内的运动过程。方法:动物实验选用SD大鼠,口服给以桔梗水提液,在24h内按时间点取血;药代动力学研究以桔梗皂苷D为检测指标,采用高效液相色谱法,使用PhenomenexC18色谱柱(100×4.6mm,5μm),乙腈:0.1%磷酸为流动相,梯度洗脱,流速1.0mL/min,进样量10μL,柱温30Ⅳ,检测波长203nm。结果:桔梗皂苷D的药代动力学参数为,T_(max)=0.69±0.44,C_(max)=21.819±1.16,AUC_((0-τ))=44.132±0.67,MRT=2.00±0.75,CL=228±0.80。结论:桔梗皂苷D的口服达峰时间较快,生物利用度较低,但已达到发挥提高其它药物疗效所需浓度。

Objective： To investigate in-body movement of kikyosaponin D in rat. Methods： animal experiment used SD rats, water extract of Platycodon grandiflorum was given orally, and blood sample was taken with 24 hours; pharmacokinetic research was carried out using kikyosaponin D as index, and HPLC method for determination, with Phenomenex C18 column and acetonitrile-0.1% phosphoric acid as mobile phase, the injection volume was 10μl, column temperature was 30Ⅳ, detection wavelength was 203 nm. Results： pharmacokinetic parameter of kikyosaponin D was T（max）=0.69±0.44, C（max）=21.819±1.16, AUC（（0-τ））=44.132±0.67, MRT=2.00±0.75, CL=228±0.80. Conclusion： oral administration of kikyosaponin D had a fast metabolism, while with lower bioavailability; however, such parameters was acceptable for Platycodon grandiflorum to achieve its effect for enhancing therapeutic of other medications.