丙戊酸钠诱导神经干细胞分化过程中肝配蛋白A3的表达

Expression of ephrin A3 during valproate induced neural stem cells differentiation

目的探讨丙戊酸钠(VPA)促进神经干细胞分化过程中肝配蛋白A3(Efna3)的表达以及Efna3在神经系统里的表达模式。方法分离培养SD大鼠海马神经干细胞,在VPA诱导分化后24 h和48 h,利用实时定量聚合酶链反应(Real-time PCR)、免疫印迹法(Western blotting)和免疫荧光技术检测Efna3的动态表达;Real-time PCR检测Efna3在成年SD大鼠各组织以及神经干细胞、星形胶质细胞和神经元中的表达水平。结果 VPA处理组与对照组相比,Efna3的表达量明显上升;Efna3在端脑和海马中呈现优势表达;Efna3在神经元中表达较高,而在星形胶质细胞中表达较低。结论 VPA促进神经干细胞分化可能与Efna3的表达上调有关。

Objective To investigate the expression of ephrin A3 （Efna3） in the process of neural stem cells differentiation induced by valproate （VPA） and the expression pattern of Efna3 in the nervous system. Methods The hippoeampal neural stem cells of SD rats were isolated and cultured. At 24 hours and 48 hours after VPA inducement, the dynamic expression of Efna3 was detected by Real-time PCR, Western blotting and immunofluoresence assay. Real-time PCR was used to detect the expression of Efna3 in various tissues, as well as neural stem cells, astrocytes and neurons of adult SD rats. Results The expression of Efna3 increased significantly in the VPA group compared to the control group. Efna3 showed dominant expression in the cerebrum and hippocampus, and its expression was higher in neurons and lower in astrocytes. Conclusion The promotion of VPA to the differentiation of neural stem cells may be related to the upregulation of Efna3.