基于网络药理学的丹参干预动脉粥样硬化分子机制的分析

A network pharmacology approach to explore the molecular mechanism of Radix Salviae on atherosclerosis

目的采用网络药理学方法探讨丹参干预动脉粥样硬化的分子机制。方法在中医药数据库和文献收集筛选丹参有效成分信息的基础上,应用PharmMapper预测化学成分的已知靶点与潜在靶点,应用DAVID数据库进行KEGG通路注释分析和GO富集分析。根据丹参的潜在靶点预测和富集分析的结果运用Cytoscape构建成分-预测靶点网络模型、成分-靶点-通路网络模型及成分-靶点-生物过程网络模型,并分析其作用机制。结果丹参的16个化学成分返回了344个潜在靶点,并获得了6条相关信号通路和28个生物过程。其中生物过程可分为四个模块——保护内皮细胞功能、抗氧化作用、抗斑块形成、调理血脂水平。结论这些发现体现了丹参对动脉粥样硬化干预的分子机制。不仅为丹参干预动脉粥样硬化的研究提供了理论依据以及为未来的相关研究提供了基础,同时对丹参等传统中药作用机制研究提供新的范式。

Aim To explore the molecular mechanism of Radix Salviae on atherosclerosis based on the network pharmacology. Methods Radix Salviae’s active compounds were collected from reference and traditional Chinese Medicine databases,and put into Pharm Mapper to get their potential targets. Then KEGG-pathway analysis and GO enrichment analysis were made by DAVID database. Finally the network was drawn and analyzed by Cytoscape with information above. Results Sixteen compounds of THP acquired 344 compound targets which also got 6 atherosclerosisrelated pathways and 28 biological processes. The biological processes can be divided into four modules—protection of endothelial cell function,antioxidant,anti-atherosclerotic plaque formation,conditioning blood lipid levels. Conclusion These findings reflect the molecular mechanism of Radix Salviae on atherosclerosis. It provides not only a theoretical basis for the study of Radix Salviae on atherosclerosis,but also a new paradigm for the study of the mechanism of traditional Chinese medicine such as Radix Salviae.