应用UPLC-ESI-MS/MS分析栀子苷在正常大鼠和急性痛风大鼠体内组织分布情况

Using UPLC-ESI-MS/MS to Analyze the Distribution of Geniposide in the Tissues of Normal Rats and Acute Gout Rats

目的:研究栀子苷在正常大鼠和急性痛风大鼠体内肝、肾、脾、脑等组织中分布情况。方法:将60只SD大鼠按照随机数字法分为正常组和模型组,每组30只,均按5g·kg-1灌胃给药丹栀逍遥散,分别于30、60、120、240、360分钟处死各组大鼠,取肝、肾、心、脑、脾、肺组织,采用超高效液相色谱-电喷雾离子化串联质谱(UPLC-ESI-MS/MS)法分析栀子苷在大鼠各组织中分布情况。结果:栀子苷在大鼠体内广泛分布,药物浓度在肾脏中最高,肝次之,脾和脑较少。正常组和模型组大鼠的肝、肾、心、脑、脾、肺组织中,栀子苷的质量浓度均在120分钟时达到最高。与正常组比较,模型组大鼠肝、肾、心、脑组织中栀子苷质量浓度在各时间点均显著升高,差异有统计学意义(P<0.05)。结论:急性痛风大鼠肝、肾等组织对栀子苷的吸收情况均优于正常大鼠,急性痛风的病理状态有利于机体对栀子苷的吸收。

Objective： To study the distribution of Geniposide in the liver,kidney,spleen,brain and other tissues of normal rats and acute gout rats. Methods： 60 SD rats were divided into normal group and odel group according to the random number method,30 rats in each group,intragastric administration of Danzhi Xiaoyao Powder in the dose of 5 g·kg^-1,the rats in each group were killed at 30,60,120,240 and 360 minutes respectively,the liver,kidney,heart,brain,spleen and lung tissues were collected,the distribution of Geniposide in each tissues of rats was analyzed by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry（ UPLC-ESI-MS/MS）. Results： Geniposide was widely distributed in rats,the concentration of the drug was the highest in the kidney,the second was the liver,and the spleen and the brain were less. In normal group and model group,the concentrations of Geniposide in liver,kidney,heart,brain,spleen and lung tissues of rats reached the highest at 120 minutes. Compared with the normal group,the concentrations of Geniposide in the liver,kidney,heart and brain tissues of the model group increased significantly at all time points,the difference was statistically significant（ P〈0. 05）. Conclusion： The absorptions of Geniposide in liver,kidney and other tissues of acute gout rats is better than those of normal rats,the pathological state of acute gout is beneficial to the absorption of Geniposide.