HPLC法测定亳州11个乡镇白芍中3个成分含量

Determination of Six Components in Paeoniae Radix Alba in 11 Towns of Bozhou by HPLC

目的：建立HPLC法同时测定亳州11个乡镇白芍中没食子酸、芍药苷和芍药内酯苷的含量。方法：选用安捷伦ZORBAXSBC18（4.6 mm×250 mm,5μm）柱子,以乙腈（A）-0.1%磷酸水（B）为流动相,进行梯度洗脱,流速为1.0 m L/min,柱温25℃,检测波长230 nm。结果：没食子酸在8.04-40.52μg/m L范围内呈线性关系,芍药苷在44.32-221.6μg/m L范围内呈线性关系,芍药内酯苷在28.4-142μg/m L范围内呈线性关系;加样回收率分别为101.1%、100.66%、99.64%,RSD值分别为1.65%、1.18%、1.58%。结论：该研究方法全面,简便准确,可用于测定亳白芍中没食子酸、芍药苷和芍药内酯苷的含量,为亳白芍的质量控制提供参考依据。

Objective：To control the quality of Paeonia lactiflora Pall,then to build a method for gallic acid monohydrate,paeoniflorin and albiflorin at the same time. Methods：Chromatography conditions：ZORBAXSBC18 column（4.6 mm×250 mm,5 μm）with themobil ephase of acetonitrile and water containing 0.1% phosphoric acid. Flow rate was 1.0 m L/min and the detection wavelength was 230 nm,the column temperature was 25 ℃. Results：The linear ranges of gallic acid monohydrate,paeoniflorin and albiflorin were 8.04-40.52 μg/m L（r=0.9998）,44.32-221.6 μg/m L（r=0.9994）and 28.4-142 μg/m L（r=0.9996）. The average recovery were 101.1%,100.66%,99.64%,with RSD 1.65%,1.18%,1.58%. Conclusion：The method is simple,sensitive,accurate and with a good way to control the quality.