摘要
B细胞永生化是一个历久弥新的研究课题,如何维持B细胞体外连续传代增殖并表达抗体的特性,对于研制治疗和诊断用抗体类药物和制品具有重要的意义。B细胞永生化的方法包括杂交瘤技术,EB病毒或SV40感染永生化以及遗传修饰法。永生化B细胞能快速有效的产生单克隆抗体,这种抗体能克服异源抗体产生的免疫排斥,是制备单克隆抗体的理想来源,成为研制治疗性单抗的重要方法。本文主要概述了B细胞永生化方法的研究进展与未来的发展方向,以供从事这方面研究的人员参考。
Methods on immortalization of B cell are always a research focus.How to keep the B cell continuous propagation and expression of antibodies plays a vital role in the development of therapeutic and diagnostic antibody drugs and products.Methods for immortalizing B cells include hybridoma technology,infection with Epstein-Barr virus or SV40 virus,and genetic modification of B cell.The immortalized B cells which can rapidly and efficiently produce monoclonal antibodies and overcome immune rejection among different species,are therefore an ideal source of monoclonal antibodies.This article mainly summarizes the recent research progresses and future direction in B cell immortalization,which could provide some references for people in this research area.
作者
王省
李坤
卢曾军
汪肖肖
谯雨
刘在新
曹轶梅
WANG Sheng;LI Kun;LU Zeng-jun;WANG Xiao-xiao;QIAO Yu;LIU Zai-xin;CAO Yi-mei(State Key Laboratory of Veterinary Etiological Biology~National Foot-and-Mouth Disease Reference Laboratory/ Lanzhou Veterinary Research lnstitute , Chinese A cademy of A gricultural Sciences ,Lanzhou 730046,Chin)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2018年第6期743-749,共7页
Chinese Veterinary Science
基金
国家自然科学基金项目(31372422)
关键词
B细胞
单克隆抗体
永生化
B cell
monoclonal antibody
immortalization
