染料木素对UVA诱导成纤维细胞急性光损伤的防护作用

Protective effect of genistein on acute light damage induced by UVA in fibroblasts cells

目的:明确染料木素对UVA诱导成纤维细胞急性光损伤的防护作用。方法:将体外培养的成纤维细胞分为空白对照组,UVA照射对照组及UVA+染料木素(0.01,0.1,1,10,100μmol/L)组。采用CCK8法测定细胞增殖情况,流式细胞术检测细胞凋亡情况,RT-PCR检测Sirt1mRNA的表达水平。结果:与UVA组比较,UVA+0.01,0.1,1,10μmol/L染料木素组细胞增值活性及Sirt1mRNA表达明显升高,细胞凋亡率降低(P<0.05);而UVA+100μmol/L染料木素,细胞凋亡率无明显差异(P>0.05);结论:0.01～10μmol/L染料木素对UVA诱导成纤维细胞的急性光损伤有防护作用。

Objective： To determine the protective effect of genistein on acute light damage induced by UVA in fibroblasts cells. Methods： Fibroblasts cells cultured in vitro were divided into the control group,UVA group and UVA＋0.01,0.1,1,10,100 μmol/L genistein group. The proliferation activity,apoptosis rate and the level of Sirt1 mR NA were detected by the cck8 experiment method,flow cytometry and RT-PCR,respectively. Results： Compared with the UVA group,the proliferation activity and the level of Sirt1 mR NA in the UVA＋ 0.01,0.1,1,10 μmol/L genistein group was significantly increased and the apoptosis rate was significantly decreased（ P〈0.05）,while there was no significant difference in apoptosis rate between the UVA ＋100 umol/L genistein group and UVA group（ P〈0.05）. Conclusion： 0.01-10 μmol/L genistein is effective in protecting the fibroblasts cells from acute light damage induced by UVA.