叶黄素对人前列腺癌PC3细胞增殖和凋亡影响的机制

Effect of Lutein on Proliferation and Apoptosis of Human Prostate Cancer PC3 Cell Line and Related Mechanism

目的探究叶黄素对人前列腺癌PC3细胞增殖和凋亡影响的作用机制,为前列腺癌预防及治疗提供新的理论依据。方法不同浓度叶黄素作用于PC3细胞,CCK8法检测细胞增殖情况;流式细胞仪检测细胞周期分布和凋亡变化;细胞划痕和Transwell实验观察细胞迁移和侵袭能力;RT-PCR和Western blot技术检测细胞中Bax、Bcl-2的mRNA水平和蛋白表达。结果叶黄素显著抑制PC3细胞的增殖,并呈时间和浓度依赖性。叶黄素可以将细胞生长阻滞在G_0/G_1期,抑制细胞迁移和侵袭;还可促进细胞凋亡,使Bcl-2表达下降,Bax表达上升。叶黄素可在转录水平上下调Bcl-2 mRNA和上调Bax mRNA。结论叶黄素抑制PC3细胞增殖并促进其凋亡,其机制可能与阻滞细胞周期、抑制细胞迁移、侵袭以及调节凋亡相关基因和蛋白表达有关。

Objective To investigate the mechanism of lutein on the proliferation and apoptosis of human prostate cancer PC3 cells, and to provide a new theoretical basis for the treatment and prevention of prostate cancer. Methods CCK8 assay was used to detect the proliferation of PC3 cells treated with different concentrations of lutein; flow cytometry was used to detect the changes of cell cycle distribution and apoptosis after 48 h; cell scratch（wound healing） and Transwell experiment was applied to observe cell migration and invasion; the mRNA and protein expression levels of Bcl-2 and Bax were detected by RT-PCR and Western blot. Results Lutein significantly inhibited the proliferation of PC3 cells in a time-and dose-dependent manner. Lutein could block the growth of PC3 cells in G0/G1 phase and inhibit cell migration and invasion; it could induce cell apoptosis, downregulate the expression of Bcl-2 and upregulate the expression of Bax. RT-PCR results showed that lutein could downregulate Bcl-2 mRNA expression and upregulate Bax mRNA expression in a dose-dependent manner. Conclusion Lutein could inhibit the proliferation and promote the apoptosis of human prostate cancer PC3 cells. The mechanism may be related to blocking cell cycle, inhibiting cell migration and invasion, as well as regulating apoptosis-related gene and protein expression.