菌落PCR在快速鉴定头癣病原菌中的临床应用

Clinical application of colony PCR in rapid detection of pathogenic fungi causing tinea capitis

目的评价菌落PCR在快速鉴定头癣病原菌中的可靠性及临床实用性。方法于2016年1月至2017年3月在无锡市第二人民医院皮肤科门诊收集儿童头癣病例17例，采用菌落PCR技术检测病原菌，同时与常规PCR及形态学鉴定结果进行比较，评价菌落PCR应用于头癣病原菌鉴定的可靠性。结果17例儿童头癣患者临床标本经培养收集菌种、菌落PCR均扩增成功，从断发或皮屑标本中取材至DNA模板制备成功耗时（3．82±0．50）d，较传统形态学鉴定（14d）明显缩短。以常规PCR鉴定结果为标准，菌落PCR菌种鉴定正确率为100％，优于传统的形态学鉴定（正确率88．2％）。结论菌落PCR可用于临床头癣病原菌的检测，是一种快速、经济、可靠的分子检测技术。

Objective To evaluate the reliability and clinical practicality of colony PCR in rapid detection of pathogenic fungi causing tinea capitis. Methods Totally, 17 children with tinea capitis were enrolled from the Department of Dermatology of Wuxi No. 2 People＇ s Hospital between January 2016 and March 2017. Colony PCR was performed to detect pathogenic fungi. The resnhs of colony PCR were compared with those of routine PCR and morphological identification, so as to evaluate the reliability of colony PCR in the identification of pathogenic fungi causing tinea capitis. Results After clinical specimens （broken hairs and scales） from the 17 patients were subjected to fungal culture, the mycelia were collected and successfully amplified by colony PCR. The time of clony PCR （mean, 3.82 ±0.50 days） for DNA template preparation was short than that of traditional morphological identification （14 days）. Based on the results of conventional PCR, the accuracy of colony PCR for fungal identification was 100%, which was superior to that of conventional PCR （88.2%）. Conclusions Colony PCR can be applied to the clinical detection of pathogenic fungi causing tinea capitis at specy level, and is a kind of rapid, economic and reliable molecular detection technique.