长链非编码RNA在儿童脓毒症中表达的初步研究

Preliminary study of the expression of long chain non-coding RNA in sepsis children

目的探讨长链非编码RNA（long non-coding RNA，lncRNA）在脓毒症患儿的表达及临床应用价值。方法2014年1月至2014年7月收集上海交通大学医学院附属上海儿童医学中心重症医学科的15例一般脓毒症患儿、7例脓毒性休克患儿及21例儿童保健科健康体检儿童（健康对照组）的外周血标本。通过实时定量聚合酶链式反应（real time-polymerase chain reaction，RT-PCR）方法检测核因子-κB通路中，与脓毒症密切相关的9个lncRNA（AK092960、LOC100192426、VHDJH、AC006230.3、AC019097.7、RP4-652L8.2、RP11-108A15.2、NKILA及AK023660）的表达情况。并进一步分析一般脓毒症组、脓毒性休克组及健康对照组患儿lncRNA表达量的差异，比较lncRNA与C-反应蛋白、降钙素原、WBC诊断脓毒症和脓毒性休克的特异性和敏感性。结果VHDJH、AC019097.7、RP4-652L8.2、RP11-108A15.2、NKILA及AK023660在脓毒症组表达较健康对照组明显升高，差异有统计学意义（P〈0.01）。其中，VHDJH、AC019097.7、RP4-652L8.2、NKILA及AK023660在脓毒性休克患儿表达量较一般脓毒症组高，差异有统计学意义（P〈0.01）。虽然VHDJH、AC019097.7、RP4-652L8.2、NKILA、AK023660诊断脓毒性休克与脓毒症的特异性及敏感性均高于C-反应蛋白、降钙素原、WBC，但差异无统计学意义（P〉0.05）。结论lncRNA中的VHDJH、AC019097.7、RP4-652L8.2、RP11-108A15.2、NKILA、AK023660可作为诊断脓毒症的潜在标记物，并可能与脓毒症的严重程度相关。

Objective To explore the expression and clinical value of long non-coding RNA （lncRNA） in sepsis children. Methods The peripheral blood samples were analyzed from 15 sepsis children （ sepsis group） ,7 septic shock children（ septic shock group） and 21 healthy children（ healthy control group）. The real time-polymerase chain reaction was used to explore the expression of 9 kinds of lncRNA （ AK092960, LOC100192426, VHDJH, AC006230. 3, AC019097.7, RP4-652L8.2, RP11-108A15.2, NKILA and AK023660） which are closely related to the nuclear factor-κB pathway of the sepsis. And further analysis of lncRNA expression between sepsis, septic shock and health children were carried out. The specificity and sensitivity of the lncRNAs compared with C-reactive protein, procalcitonin and WBC for identification of patients with sepsis or septic shock were also evaluated. Results The expression of VHDJH,AC019097.7, RP4-652L8.2,RPll-108A15.2, NKILA and AK02366 in the sepsis group were significantly higher than those in the healthy control group （ P 〈 0. 01 ）. Among them, the expression of VHDJH, AC019097.7, RP4-652LS. 2 ,NKILA and AK023660 in the septic shock group were higher than those in the sepsis group （P 〈0. 01 ）. Although the specificity and sensitivity of VHDJH, AC019097. 7, RP4-652L8.2, NKILA and AK023660 were higher than C-reactive protein,procalcitonin and WBC for sepsis and septic shock, respectively, there was no significant difference statistically （ P 〉 0. 05 ）. Conclusion VHDJH, AC019097. 7, RP4-652L8. 2, RPll-108A15.2 ,NKILA and AK023660 could be the potential diagnostic biomarkers of sepsis and might reflect its severity.