基于Illumina MiSeq高通量测序分析黄芪内生真菌的多样性

Analysis of Diversity of Endophytic Fungi in Astragali Radix by Illumina MiSeq High-throughput Sequencing Technology

目的：分析黄芪内生真菌群落多样性,为筛选菌种进行发酵炮制转化黄芪中活性成分的定向炮制工艺研究提供参考依据。方法：应用Illumina MiSeq高通量测序技术测定黄芪内生真菌的18S rDNA NS1-Fung区域序列并进行物种丰度等生物信息分析。结果：获得有效序列29 677条,操作分类单元188个,测序数量接近于饱和,测序数据量合理。黄芪内生真菌主要分布于驼孢锈菌属（Hemileia）,赤霉菌属（Gibberella）,粪盘菌属（Ascobolus）,假裸囊菌属（Pseudogymnoascus）和曲霉属（Aspergillus）共5个属。结论：黄芪内生真菌群落多样性较低,Hemileia和Gibberella是黄芪内生真菌的优势种群。

Objective： This study aimed to analysis the diversity of endophytic fungal communities in Astragali Radix,and provide a reference for study on the directional processing technology of active ingredients in this herb. Method： The 18 S rDNA NS1-Fung region of endophytic fungal communities in Astragali Radix was sequenced by Illumina MiSeq high-throughput sequencing technology, abundance of species and other bioinformation were analyzed. Result： The numbers of effective sequences and operational taxonomic units for samples were 29 677 and 188, respectively. The number of sequencing was close to saturation, and the sequencing data volume was reasonable. The main endophytic fungi of Astragali Radix belonged to 5 genera,such as Hemileia,Gibberella,Ascobolus,Pseudogymnoascus and Aspergillus. Conclusion： By and large,the diversity of endophytic fungi in Astragali Radix is low. Hemileia and Gibberella are the dominant endophytic fungi in Astragali Radix.