沉香超微粉与山沉香超微粉药效学对比分析

Comparison of Pharmacodynamics Between Aquilariae Lignum Resinatum Ultrafine Powder and Syringa pinnatifolia var. alashanensis Ultrafine Powder

目的：研究沉香超微粉、原粉抗心肌缺血、耐缺氧、镇痛与镇静的药理学作用,并与山沉香超微粉作对比研究。方法：冠脉结扎方法制造大鼠心肌缺血模型,并观察沉香与山沉香各给药组大鼠心梗面积,心电图,血清肌酸激酶（CK）,超氧化物歧化酶（SOD）,乳酸脱氢酶（LDH）;钠石灰法观察沉香与山沉香各给药组小鼠存活时间;乙酸扭体法和戊巴比妥钠协同法观察沉香与山沉香各给药组对小鼠的镇痛、镇静作用。结果：沉香超微粉、原粉和山沉香超微粉均有不同程度抗大鼠心肌缺血作用：可降低大鼠血清CK,LDH的活性,提高血清SOD的活性,并能降低心肌缺血的面积,明显改善心电图（P〈0.05,P〈0.01）,且沉香超微粉0.75,0.375 g·kg^-1剂量组明显优于沉香原粉（P〈0.05）;相同剂量沉香超微粉与山沉香超微粉作用相当;沉香、山沉香各给药组对小鼠缺氧状态下存活时间均有延长作用（P〈0.05）,相同剂量下山沉香超微粉作用优于沉香超微粉（P〈0.05）;沉香和山沉香超微粉组可明显延长小鼠扭体潜伏期（P〈0.05）,并减少扭体次数（P〈0.05）,同剂量下山沉香超微粉与沉香超微粉组作用相当;山沉香和沉香超微粉对阈剂量戊巴比妥钠致小鼠睡眠时间均有明显延长作用（P〈0.05）。山沉香超微粉与对应剂量沉香超微粉组作用相当。结论：沉香超微粉、原粉与山沉香超微粉均有抗心肌缺血、耐缺氧、镇痛、镇静等作用,且同剂量下沉香超微粉作用优于其原粉,山沉香超微粉作用相当于对应剂量的沉香超微粉。

Objective： To investigate the pharmacological effects of Aquilariae Lignum Resinatum ultrafine powder and fine powder about its anti-myocardial ischemia,anti-anoxia,analgesia and sedation effects,and compare with Syringa pinnatifolia var. alashanensis ultrafine powder. Method： Coronary artery ligation method was used to establish models of myocardial ischemia in rats. The myocardial infarction area,electrocardiogram,serum creatine kinase（ CK）,superoxide dismutase（ SOD） and lactate dehydrogenase（ LDH）were observed in each group. Survival time of mice in both Aquilariae Lignum Resinatum and S. pinnatifolia var.alashanensis groups was observed by Soda Lime method. Acetic acid writhing method and pentobarbital sodium synergetic methodwere used to observe the analgesic and sedative effects of Aquilariae Lignum Resinatum and S.pinnatifolia var. alashanensis in mice. Result： Aquilariae Lignum Resinatum ultrafine powder,fine powder and S. pinnatifolia var. alashanensis ultrafine powder had different degrees of effects on myocardial ischemia in rats：reduced the contents of serum CK and LDH,increased the content of SOD in serum,decreased the myocardial ischemia area and ameliorated the ECG performance（ P〈0. 05, P〈0. 01）. Aquilariae Lignum Resinatum ultrafine powder 0. 75,0. 375 g·kg^-1 dose groups were significantly better than the fine powder（ P〈0. 05）; the same dose of Aquilariae Lignum Resinatumu ltrafine powder and S. pinnatifolia var. alashanensis ultrafine powder showed equivalent efficacy; the survival time of mice under hypoxia was prolonged in various groups（ P〈0. 05）,and the effect of S. pinnatifolia var. alashanensis ultrafine powder was better than that of Aquilariae Lignum Resinatum ultrafine powder at the same dose（ P〈0. 05）; the group of Aquilariae Lignum Resinatum ultrafine powder and S. pinnatifolia var. alashanensis ultrafine powder could significantly prolong the mice writhing latency（ P〈0. 05） and reduce writhing times（ P〈0. 05）,with equivalent efficacy at the same dose. Aquilariae Lignum Resinatum ultrafine powder and S. pinnatifolia var. alashanensis ultrafine powder could evidently prolong the sleep time of mice with threshold doses of pentobarbital sodium（ P〈0. 05）,showing equivalent efficacy at the same dose. Conclusion： Aquilariae Lignum Resinatum ultrafine powder, fine powder and S. pinnatifolia var.alashanensis ultrafine powder had the effects of anti-myocardial ischemia,anti-hypoxia,analgesia,and sedation. In the same dosage,the effects of Aquilariae Lignum Resinatum ultrafine powder were better than those of the fine powder,but equivalent with those of S. pinnatifolia var. alashanensis ultrafine powder.