娄彻氏链霉菌木聚糖酶水解玉米芯高温蒸煮液高效制备低聚木糖

High-producing Xylanase by Streptomyces rochei Hydrolysis High-temperature Cooking Liquid of Corncob

系统考察具有高度催化特异性的娄彻氏链霉菌L10904所产木聚糖酶在工业制备低聚木糖中的应用。以玉米芯高温蒸煮喷爆液为水解底物,通过单因素及Box-Benhnken响应面试验优化低聚木糖的生产工艺。结果显示,最佳酶解条件:反应体系pH 5.3,水解时间7.0 h,加酶量10.0 U/m L,水解温度59℃。较优化前木二、木三糖的还原糖占比提高了32.96%,具有较好的应用前景。

Hydrolysis conditions of XOS preparation of high-temperature cooking liquid were optimized, through single factor experiment and response surface analysis. Qualitatively study the polysaccharide in high-temperature cooking liquid, conduct initial degradation experiments using the methods of enzymatic hydrolysis, exploring the transforming relationship between xylan and XOS. The optimal hydrolysis conditions were： the initial pH 5.3, hydrolysis time 7.0 h, enzyme concentration 10.0 U/m L, hydrolysis temperature of 59 ℃. It was found that enzymatic degradation can degrade xylan producing XOS, production with xylobiose and xylotriose based; have a higher proportion of hybrid glycans.