E-钙黏蛋白与波形蛋白在胃癌中的表达及意义

Expressions and significances of E-cadherin and Vimentin in gastric cancer

目的探讨胃癌患者中E-钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)的表达及其临床意义。方法 60例胃癌患者,取手术切除胃癌标本及癌旁组织各60份分别为胃癌组和癌旁组,非肿瘤性胃切除标本20份为对照组,采用免疫组织化学法检测3组E-cadherin及Vimentin阳性表达情况,Spearman法分析胃癌组E-cadherin和Vimentin阳性表达的相关性,分析胃癌组E-cadherin和Vimentin阳性表达与临床病理特征的关系。结果胃癌组E-cadherin阳性表达率(28.3%)低于癌旁组(46.7%)和对照组(80.0%)(P<0.05),癌旁组低于对照组(P<0.05);胃癌组Vimentin阳性表达率(70.0%)高于癌旁组(50.0%)和对照组(35.0%)(P<0.05),癌旁组高于对照组(P<0.05);Spearman关联分析结果显示,胃癌组E-cadherin和Vimentin表达呈负相关(r=-0.476,P<0.001),无淋巴结转移者E-cadherin阳性表达率(40.0%)高于有淋巴结转移者(12.0%)(P<0.05),TNM分期Ⅲ~Ⅳ期、肿瘤低分化、有淋巴结转移者Vimentin阳性表达率(82.1%、85.2%、84.0%)均高于TNM分期Ⅰ~Ⅱ期、肿瘤中高分化、无淋巴结转移者(47.6%、57.6%、60.0%)(P<0.05)。结论胃癌组织中E-cadherin表达降低,Vimentin表达升高,二者与胃癌的侵袭及转移有关。

Objective To investigate the expressions and clinical significances of E-cadherin and Vimentin in gastric cancer.Methods Gastric cancer tissues（cancer group）and paracancerous tissues（paracancerous group）were collected from 60 patients with gastric cancer,while noneoplastic gastric tissues were collected from 20 patients as control group.The positive expressions of E-cadherin and Vimentin were detected by immunohistochemistric method.Spearman correlation analysis method was adopted to analyze the correlation between E-cadherin and Vimentin and between the clinicopathological features and the positive expressions of E-cadherin and Vimentin in cancer group.Results The positive expression of E-cadherin was significantly lower in cancer group（28.3%）than that in paracancerous group（46.7%）and control group（80.0%）（P〈0.05）,and in paracancerous group than that in control group（P〈0.05）.The positive expression of Vimentin was significantly higher in cancer group（70.0%）than that in paracancerous group（50.0%）and control group（35.0%）（P〈0.05）,and in paracancerous group than that in control group（P〈0.05）.Spearman correlation analysis showed that E-cadherin was negatively correlated with Vimentin in cancer group（r=-0.476,P〈0.001）.The positive rate of E-cadherin was significantly higher in patients with non-metastasis of lymph node（40.0%）than that in those with lymph node metastasis（12.0%）（P〈0.05）,and the positive rates of Vimentin were significantly higher in patients in TNM stageⅢ-Ⅳ（82.1%）,with poor differentiation（85.2%）and lymph node metastasis（84.0%）than those in patients in TNM stageⅠ-Ⅱ（47.6%）,with moderate to well differentiation（57.6%）and non-metastasis of lymph node（60.0%）（P〈0.05）.Conclusion E-cadherin is lowly expressed and Vimentin is highly expressed in gastric cancer tissues,which is correlated with the invasion and metastasis of gastric cancer.