黑茶茶褐素与茶多糖对脂肪酶的抑制作用

Study of inhibition of black tea theabrownin and tea polysaccharides on lipase

为探讨黑茶茶褐素与茶多糖对脂肪酶的抑制作用,选择不同地区相同级别的黑茶为原料,提取茶褐素与茶多糖,采用对硝基苯酚法,分析茶褐素与茶多糖对脂肪酶活性的抑制率,并探讨各黑茶茶褐素与茶多糖对脂肪酶的抑制机理。结果表明:黑茶茶褐素与茶多糖对脂肪酶具有显著的抑制作用,普洱茶茶褐素与茶多糖IC50最小,分别为25.96,47.57mg/mL,抑制效果最好;其次为六堡茶;抑制效果最差的为茯砖茶褐素与茶多糖;康砖茶褐素与茶多糖抑制效果介于六堡茶与茯砖茶之间。各黑茶茶褐素的抑制类型均为可逆竞争性与非竞争性混合型,其中六堡茶接近于竞争性抑制类型;普洱茶、六堡茶与康砖茶多糖的抑制类型为可逆竞争性与非竞争性混合型,茯砖茶多糖抑制类型为可逆竞争性抑制。

In order to investigate the inhibition of lipase by theabrownin and polysaccharide of black tea,the theabrownin and polysaccharides were extracted from the same black tea in different regions.The inhibition rate of lipase activity of theabrownin and polysaccharide was analyzed by p-nitrophenol method,and the inhibitory mechanism of theabrownin and polysaccharide on lipase was discussed.The results showed that the inhibition of lipase by theabrownin and polysaccharide of black tea was significant.The IC50 of Pu-er theabrownin and polysaccharide were 25.96 and 47.57 mg/mL,respectively,which were the minimum,and the inhibitory effect was the best,secondly for liupu tea.The worst inhibitory effect was that the theabrownin and polysaccharide of fu-brick.The inhibition effects of theabrownin and polysaccharide of Kang brick were between those of the liupu tea and fu-brick.The inhibition types of theabrownin of each black tea were the complex of reversible competitive and noncompetitive,while the liupu tea was closed to the competitive inhibition type.The inhibition types of polysaccharide of Pu-er tea,liupu tea and Kang brick were the complex of reversible competitive and noncompetitive,while the polysaccharide of fu-brick was reversible competitive inhibition.