聚乙烯亚胺修饰的氧化石墨烯对人胰岛淀粉样蛋白聚集的抑制作用

Polyethyleneimine Functionalized Graphene Oxide Against hIAPP Amyloid Aggregation

利用共价结合的方法在氧化石墨烯(GO)表面修饰聚乙烯亚胺(PEI),得到了稳定的GO-PEI复合物.透射电子显微镜和圆二色谱实验结果显示,对于人类胰岛淀粉样多肽(hIAPP)的纤维化聚集GO-PEI比GO有更强的抑制能力.结合Th T荧光和原子力显微镜实验结果发现,在hIAPP聚集过程的成核初期加入GO-PEI有最好的抑制效果,在纤维化过程的生长期加入GO-PEI能部分抑制hIAPP纤维的形成,但GO-PEI不能使成熟的hIAPP纤维解聚集.

Preventing human islet amyloid polypeptide（hIAPP） fibrillogenesis has been a primary strategy in development of therapeutic drugs for type Ⅱ diabetes. In this study,we prepared stable GO-PEI complex by modification of polyetherimide（PEI） on the surface of graphene oxide（GO） using covalent binding method.The results of transmission electron microscopy（TEM） and circular dichroism（CD） show that GO-PEI is more efficient than GO in inhibiting hIAPP fibril formation. The Th T fluorescence assay and atomic force microscopy（AFM） image data show that GO-PEI is the most effective for the inhibition of hIAPP fibrillation when it is added at the initial stage of hIAPP aggregation,and less effective when added in the growing period of hIAPP aggregation,but the inhibitor disables to disrupt the matured hIAPP fibrils. The findings may provide information valuable to the research and development of GO-based inhibitors.