PVA包埋丝孢酵母TX1处理含酚废水试验研究

Study on phenolic wastewater treatment by Trichosporon sp. X1 immobilized by PVA

为了提高微生物对苯酚降解的耐受浓度,利用固定化细胞技术在水处理中的优势,将具有高效降酚菌株TX1进行PVA包埋,考察该固定化细胞的降酚效果及其在实际煤气洗冷废水处理中的应用效果。试验结果表明:在气升式生物反应器中处理模拟含酚废水,固定化细胞TX1在初始苯酚浓度高达5 000 mg/L时,运行10 d后苯酚去除率达94%,容积负荷高达2.857 kg/(m^3·d),而游离细胞TX1达到该去除率时,进水苯酚浓度浓度需小于1 500 mg/L,说明固定化细胞体系更有利于处理高浓度含酚废水。将游离细胞和固定化细胞组合用于处理煤气洗冷废水,连续运行15 d后的挥发酚去除率达90%。气相色谱-质谱分析结果表明,该组合方法不仅增强了原有系统抗冲击负荷的能力,而且还提高了废水的处理效果,在工程上具有一定的实用价值。

The paper took advantage of immobilized cell technology to improve the tolerance of microbial degradation of phenol,the highly effective phenol-degrading strain of Trichosporon sp. X1 was immobilized by PVA and this application in the degradation of phenol and the treatment of actual gas-washing wastewater was also considered. The results showed that under the air-lift bioreactor with semi-continuous operation,the bioreactor was performed at a inlet phenol concentration as high as 5 000 mg/L,the phenol in the effluent was observed to reach a stable level with removal efficiency at around 94% after running 10 d and the volumetric loading up to 2.857 kg/（ m^3·d）. In contrast,free-cell system was suitable to treat the wastewater of phenol concentration less than 1 500 mg/L. This indicated that immobilized cells system was more conducive to treat the phenolic wastewater of high concentration. the combination system of the free-cell and immobilized-cell was used to treat the cold-gas washing wastewater. The phenol removal rate reached 90% after running 15 d,and GC-MS analysis results of effluent showed that the combination technology could enhance the capacity of the original system shock load and improve efficiency of the water treatment. In addition,this combination system could take a reference for the actual cold-gas washing wastewater treatment engineering.