一种双因子控释牙周再生支架材料的构建

Preparation of dual factor-released scaffolds for periodontal regeneration

目的:构建一种能够控释血小板源性生长因子(platelet-derived growth factor,PDGF)和辛伐他汀(simvastatin,SMV)的生物支架材料,检测两种因子的缓释特征。方法:以静电纺丝技术制备SMV/胶原/聚己内酯膜状支架,进行接触角检测;然后以层层自组装技术在膜状支架表面构建PDGF缓释层,形成PDGF表层-SMV芯层的双因子负载支架材料。进行体外释放实验,分别采用ELISA法和紫外分光光度计检测支架材料释放的PDGF和SMV浓度,分析PDGF和SMV释放特征。结果:胶原能够显著改善SMV/聚己内酯膜状支架的亲水性。体外释放实验结果显示,PDGF释放较早,在3~13 d达到稳定释放期;SMV释放较晚,在6~21 d为稳定释放期。结论:构建PDGF表层-SMV芯层结构的双因子支架材料,实现PDGF和SMV的序贯缓释,有望作为一种用于牙周组织再生的双因子控释系统。

Objective： To prepare dual factor-loaded scaffolds for controlled release of platelet-derived growth factor（ PDGF） and simvastatin（ SMV） and to evaluate the release properties of this formulation. Methods： SMV was encapsulated in collagen/polycaprolactone membranes using the electrospining method and surface wettability of the membranes was determined by static contact angle measurement. Then,PDGF was assembled onto the surface of electrospun membranes using the electrostatic layer-by-layer coating method. The in vitro release profiles of PDGF and SMV were determined by an enzyme linked immunosorbent assay（ ELISA） kit and an ultraviolet-visible spectrometer,respectively. Results： Reduction of water contact angle showed good hydrophilicity of the polycaprolactone membrane,which increased in the presence of collagen. The in vitro release analysis demonstrated a sequential release profile,which was characterized by the fast release of PDGF with sustained concentration from day-3 to day-13,followed by the slow release of SMV with sustained concentration from day-6 to day-21. Conclusion： Dual factor-released membrane scaffolds were successfully fabricated by encapsulating PDGF and SMV with distinct shell-core structure. The combination and sequential release of PDGF and SMV could be application in periodontal tissue engineering.