Rab1A对胶质瘤细胞增殖的影响及其机制研究

The effects of Rabl A on the proliferation of glioma cells and related mechanisms

目的探讨Rab1A基因对胶质瘤U251和U87细胞增殖能力的影响及其相关机制。方法通过si-LentFect分别将Negative control siRNA、RablA siRNA转染入胶质瘤细胞；采用Westernblot法检测转染前、后胶质瘤细胞RablA蛋白的表达；应用CCK-8细胞增殖实验和EdU实验检测RablA siRNA对胶质瘤U251和U87细胞增殖的影响；采用Westernblot法分析RablA影响胶质瘤增殖的分子机制。结果下调Rab1A基因的表达对胶质瘤U251和U87细胞增殖能力具有明显抑制作用；Rab1A能够正调控核糖体s6蛋白激酶（S6Kinase，P70S6K）。结论Rab1AsiRNA能够有效抑制胶质瘤U251和U87细胞中Rab1A蛋白的表达。Rab1A基因可能通过正调控P70S6K促进胶质瘤U251和U87细胞增殖能力。

Objective To investigate the effects of RablA gene on the proliferation of glioma U251 and U87 cells and related mechanisms. Methods Negative control siRNA and RablA siRNA were transfected into glioma cells by si- LentFect. The levels of RablA protein in glioma cells were detected by Western blotting before and after transfection. The effects of RablA siRNA on the proliferation of U251 and U87 cells were detected by CCK - 8 assay and EdU assay. The molecular mechanism of RablA affecting glioma proliferation was analyzed by Western blotting. Results Down - regulated expression of RablA gene resulted in remarkably inhibitory effects on the proliferation of glioma U251 and U87 cells. RablA was able to regulate P70S6K. Conclusions RablA siRNA can effectively inhibit the expression of RablA protein in glioma U251 and U87 cells. RablA gene may promote the proliferation of glioma U251 and U87 cells by regu-lating P70S6K.