2615例女性患者人乳头瘤病毒感染基因PCR-反向点杂交分型技术检测的研究

Study on Infective Gene Determination of 2 615 Patients of Human Papillomavirus by PCR-reverse Dot Hybridization Technique

目的:研究女性患者人乳头瘤病毒(HPV)感染基因PCR-反向点杂交分型技术的检测,为临床HPV分型及其治疗提供参考。方法:选取2015年10月—2017年11月间就诊的有性生活史或已婚女性受试者2 615例资料,对资料中采用聚合酶链式反应(PCR)反向点杂交法检测的女性宫颈分泌物中25种HPV分型结果,分析HPV亚型分类感染情况及其分型,探讨PCR-反向点杂交分型技术在宫颈癌早期筛查中的应用价值。结果:2 615例受试女性中HPV亚型检出率为28.60%,在25型HPV分型中高危型HPV 52亚型的感染率为最高(占6.50%),单重感染占20.23%,多重感染占8.37%;不同年龄段患者中>20岁女性的HPV感染率占86.49%,且不同年龄组女性患者中HPV感染率经比较其差异有统计学意义(P<0.05)。结论:临床HPV感染基因的PCR-反向杂交分型技术检测分型,可有效检测高危型及多重型HPV感染,对宫颈鳞状细胞癌的早期诊断与普查具有重要的应用价值。

Objective： To study an infective gene determination of patients of human papillomavirus（HPV） by pcrreverse dot hybridization technique, and to provide a reference for clinical HPV typing and its treatment. Methods： 2615 cases of sexual life history or married women were selected from October 2015 to November 2017. In the analysis,25 kinds of human papillomavirus（HPV） in female cervical secretions were detected by polymerase chain reaction（PCR） reverse dot blot, and the classification of HPV subtypes was analyzed so as to explore the application value of PCR-reverse dot blot typing in early screening of cervical cancer. Results： the detection rate of HPV subtype in 2 615 subjects was 28.60%. In type 25 HPV type, the high risk HPV 52 subtype infection rate was the highest（6.50%）, the single infection accounted for 20.23%, and the multiple infection accounted for 8.37%. The HPV infection rate of the women in the different age groups was 86.49%, and the HPV infection rate in the women of different age groups was HPV.The difference was statistically significant（P〈0.05）. Conclusion： The detection of HPV infection gene by PCR-reverse cross typing can effectively detect high risk and multiple HPV infection. It has great value for the early diagnosis and survey of cervical squamous cell carcinoma.