消栓饮对深静脉血栓形成兔外周血IL-6、IL-8的影响

Effect of Xiaoshuan decoction on peripheral interleukin-6 and interleukin-8 in rabbits with deep venous thrombosis

目的:通过检测在消栓饮干预下深静脉血栓(DVT)兔模型IL-6、IL-8不同时段的变化水平,初步探讨消栓饮治疗深静脉血栓的作用机制。方法:将45只新西兰兔建立DVT动物模型,随机分为3组,即消栓饮组、低分子肝素钠组、0.9%氯化钠注射液组,分别于术后第1天给予消栓饮、低分子肝素钠、0.9%氯化钠注射液治疗,连续治疗1周,分别于第1、3、7天给药4h后检测各组IL-6、IL-8水平。结果:与0.9%氯化钠注射液组比较,消栓饮组与低分子肝素钠组在术后同期的检测指标均下降(P<0.05);与术后第3天比较,3组术后第1、7天检测指标水平均下降(P<0.05);消栓饮组与低分子肝素钠组术后同期检测指标比较,差异无统计学意义(P>0.05)。结论:消栓饮通过减少深静脉血栓外周血IL-6、IL-8炎性因子的释放水平,从而抑制炎症反应,对深静脉血栓形成有防治作用。

Objective： To investigate the changes in interleukin-6（ IL-6） and interleukin-8（ IL-8） in different periods of time in rabbits with deep venous thrombosis（ DVT） treated by Xiaoshuan decoction and the mechanism of action of Xiaoshuan decoction in the treatment of DVT. Methods： A total of 45 New Zealand rabbits were used to establish an animal model of DVT and then randomly divided into Xiaoshuan decoction group,low-molecular-weight heparin（ LMWH） group,and 0. 9% sodium chloride injection group. These groups were treated with Xiaoshuan decoction,LMWH,and 0. 9% sodium chloride injection,respectively,since day 1 after surgery. The course of treatment was one week. On days 1,3,and 7,the levels of IL-6 and IL-8 were measured at 4 hours after administration. Results： Compared with the 0. 9% sodium chloride injection group,the Xiaoshuan decoction group and the LMWH group had significant reductions in the above indices at each time point after surgery（ P〈0. 05）. In all groups,the values on days 1 and 7 after surgery were significantly lower than those on day 3（ P〈0. 05）. There were no significant differences in these indices between the Xiaoshuan decoction group and the LMWH group at each time point after surgery（ P〉0. 05）. Conclusion： Xiaoshuan decoction can reduce the levels of peripheral IL-6 and IL-8,inhibit inflammatory response,and thus help with the prevention and treatment of DVT.