姜黄素缓解阿霉素诱导的大鼠局灶节段性肾小球硬化

Curcumin Alleviated Adriamycin Induced Focal Segmental Glomerulosclerosis in Rats

目的:局灶节段性肾小球硬化(focal segmental glomerulosclerosis,FSGS)是临床上常见的一种肾脏疾病,最终可进展至终末期肾病(end-stage renal disease,ESRD)。姜黄素是一种多酚天然产物,从姜黄的根茎中分离出来,具有抗炎和抗氧化的作用。本项研究探索姜黄素对阿霉素诱导的FSGS大鼠的肾脏保护作用。方法:使用阿霉素尾静脉注射(10 mg/kg)诱导FSGS大鼠模型,实验组每日予姜黄素(200 mg/kg)治疗,模型组单纯给予生理盐水。6周后处死大鼠,收集血液,尿液和肾脏标本以做进一步的研究。结果:姜黄素能够激活阿霉素诱导的FSGS大鼠Nrf2和Sirt1基因,进而抑制ROS和炎症因子的产生,延缓纤维化进展,改善肾脏的结构和功能病变。结论:姜黄素可以缓解阿霉素诱导的FSGS大鼠病变进展,其可能的作用靶点为Sirt1和Nrf2两个基因,通过激活Sirt1和Nrf2来抑制炎症反应和氧化应激,进而延缓FSGS的病变进展。

Objective： Focal segmental glomerulosclerosis（ FSGS） is a common primary glomerular disease,and it ultimately could develop into end-stage renal disease（ ESRD）. Curcumin is a natural product of polyphenols,isolated from the rhizome of turmeric,with anti-inflammatory and anti-oxidative effects. This study explored the protective effect of curcumin on Adriamycin-Induced FSGS animal model. Methods： Adriamycin nephropathy was induced by Adriamycin（ 10 mg/kg body weight,diluted in normal saline） via a tail vein on day 0. The experimental group was treated with curcumin（ 200 mg/kg body weight,diluted in normal saline） daily,and the model group was given pure saline alone. Rats were sacrificed after 6 weeks and blood,urine and kidney were collected for further study. Results： Curcumin could activate the Nrf2 and Sirt1 in Adriamycin-Induced FSGS animal model,then inhibit the production of ROS and inflammatory factor,ultimately delay the progress of renal fibrosis and improve the renal structure and functional lesions. Conclusion： Curcumin could relieve the progress of Adriamycin-Induced FSGS animal model. It may through activating Nrf2 and Sirt1 to inhibit inflammatory response and oxidative stress,thus delaying the progression of FSGS.