摘要
Previous studies have shown that electroacupuncture（EA） promotes recovery of motor function in Parkinson＇s disease（PD）. However the mechanisms are not completely understood. Clinically, the subthalamic nucleus（STN） is a critical target for deep brain stimulation treatment of PD, and vesicular glutamate transporter 1（VGlu T1） plays an important role in the modulation of glutamate in the STN derived from the cortex. In this study,a 6-hydroxydopamine（6-OHDA）-lesioned rat model of PD was treated with 100 Hz EA for 4 weeks. Immunohistochemical analysis of tyrosine hydroxylase（TH） showed that EA treatment had no effect on TH expression in the ipsilateral striatum or substantia nigra pars compacta,though it alleviated several of the parkinsonian motor symptoms. Compared with the hemi-parkinsonian rats without EA treatment, the 100 Hz EA treatment significantly decreased apomorphine-induced rotation and increased the latency in the Rotarod test. Notably, the EA treatment reversed the 6-OHDA-induced down-regulation of VGlu T1 in the STN. The results demonstrated that EA alleviated motor symptoms and up-regulated VGlu T1 in the ipsilateral STN of hemi-parkinsonian rats, suggesting that up-regulation of VGlu T1 in the STN may be related to the effects of EA on parkinsonian motor symptoms via restoration of function in the cortico-STN pathway.
Previous studies have shown that electroacupuncture（EA） promotes recovery of motor function in Parkinson＇s disease（PD）. However the mechanisms are not completely understood. Clinically, the subthalamic nucleus（STN） is a critical target for deep brain stimulation treatment of PD, and vesicular glutamate transporter 1（VGlu T1） plays an important role in the modulation of glutamate in the STN derived from the cortex. In this study,a 6-hydroxydopamine（6-OHDA）-lesioned rat model of PD was treated with 100 Hz EA for 4 weeks. Immunohistochemical analysis of tyrosine hydroxylase（TH） showed that EA treatment had no effect on TH expression in the ipsilateral striatum or substantia nigra pars compacta,though it alleviated several of the parkinsonian motor symptoms. Compared with the hemi-parkinsonian rats without EA treatment, the 100 Hz EA treatment significantly decreased apomorphine-induced rotation and increased the latency in the Rotarod test. Notably, the EA treatment reversed the 6-OHDA-induced down-regulation of VGlu T1 in the STN. The results demonstrated that EA alleviated motor symptoms and up-regulated VGlu T1 in the ipsilateral STN of hemi-parkinsonian rats, suggesting that up-regulation of VGlu T1 in the STN may be related to the effects of EA on parkinsonian motor symptoms via restoration of function in the cortico-STN pathway.
基金
supported by the Beijing Municipal Science and Technology Commission (Z161100002616007)
National Key Research and Development Program (2016YFC1306300)
the Major Program of the National Natural Science Foundation of China (81527901)
the Natural Science Foundation of Beijing Municipality (7082008)
