肝癌组织和癌旁组织中CD8＋CD127＋T淋巴细胞的表达及意义

Expression and Significance of CD8^＋ CD127^＋ T Lymphocytes in Hepatocellular Carcinoma and Paracancerous Tissues

目的：研究乙肝相关性肝癌组织、癌旁慢乙肝肝组织和癌旁肝硬化肝组织中CD8＋T淋巴细胞表面CD127表达水平的变化及其意义.方法：流式细胞术检测30例乙肝相关性肝癌组织、15例癌旁慢乙肝肝组织、15例癌旁肝硬化肝组织和15例正常对照肝组织中CD8^＋T淋巴细胞CD127的表达.结果：肝癌组肝组织中CD8^＋CD127^＋T淋巴细胞显著低于正常对照组及慢乙肝组肝组织中的水平,差异有统计学意义（P均〈0.05）;CD8^＋CD127^＋T淋巴细胞水平在慢乙肝组、 肝硬化组和肝癌组依次减低,但肝硬化组和肝癌组差异均无统计学意义（P〉0.05）.结论：该研究表明肝癌中主要抗肿瘤效应细胞水平下降,并提示在慢乙肝-肝硬化-肝癌的动态进展过程中,肝组织中CD8^＋T淋巴细胞表面CD127表达逐渐下降.

Objective： To study the changes and significance of CD127 on the surface of CD8^＋ T lymphocytes in HBV-related hepatocellular carcinoma, adjacent normal tissue （chronic hepatitis B）and adjacent non-cancerous liver cirrhosis （cirrhosis）. Methods：Flow cytometry was used to detect the CD127 expression on CD8^＋ T lymphocytes from postoperative liver tissues which were obtained respectively from 30 patients with HBV-related hepatocellular carcinoma, 15 patients with chronic hepatitis B, 15 patients with cirrhosis and 15 normal controls. Results： The level of CD8^＋ CD127^＋ T cells in hepatocellular carcinoma group was significantly lower than that in normal control group （β〈0.01） and chronic hepatitis B group （P〈0.05）. The level of CD8^＋ CD127^＋ T cells in chronic hepatitis B group was lower than that in normal control group, which were gradually decreased in chronic hepatitis B group, cirrhosis group and hepatocellular carcinoma group. However, there was no difference between liver cirrhosis group and hepatocellular carcinoma group （P〉0.05）. Conclusion： During the dynamic changes of chronic hepatitis B-cirrhosis-liver cancer, the expression of CD127 on CD8^＋ T cells in liver gradually decreased, which may be related to the dynamic deterioration of the diseases.