TREM-1与ICAM-1在内毒素致兔ARDS中的表达及其意义

The expression and significance of TREM-1 and ICAM-1 in acute respiratory distress syndrome induced by endotoxin in rabbits

目的:本实验以脂多糖(LPS)静脉注射的方法复制兔急性呼吸窘迫综合征(ARDS)模型,通过观察模型动物的病理生理变化,探讨ARDS的发病机理及防治方法。方法:21只新西兰白兔被随机分成3组,分别为对照组(A组)、致伤组(B组)和干预组(C组)。B组一次性静注脂多糖(剂量为720μg/kg)建立实验动物模型;C组除同等剂量脂多糖外,同时给予复方苦参注射液(剂量为4ml/kg)进行干预;A组静注同等容量0.9%NaCl注射液。不同时间点观察PaO_(2、)PaO_2/FiO_2变化,测定肺组织湿/干重比、肺组织髓样细胞触发性受体-1(TREM-1)及细胞间黏附因子-1(ICAM-1)的表达,光镜下观察各组肺组织病理变化。结果:A组各项指标基本正常;B组致伤后PaO_(2、)PaO_2/FiO_2下降,湿/干重比增加,TREM-1及ICAM-1表达明显增加,光镜下可见肺泡间隔显著增厚、炎细胞浸润;C组损伤明显轻于B组。结论:一次性静注LPS(720μg/kg)可复制兔ARDS动物模型,TREM-1和ICAM-1在模型动物损伤组中表达明显增加,复方苦参具有一定保护作用。

Objective：The acute respiratory distress syndrome（ARDS）models of rabbits were replicated with lipopolysaccharide（LPS）.By observing a series of pathophysiological changes,we explored the pathogenesis of ARDS in order to provide theoretical reference for clinical prevention and treatment of ARDS.Method：Twenty-one New-Zealand white rabbits were randomly assigned to A.B.C groups.There were control group,injury group and therapeutic group.Group B was injected LPS（720μg/kg）intravenously to replicate ARDS models.Group C was replicated by the same way.Fufangkushen（4ml/kg）was injected for treatment simultaneously.Group A was given saline of equal volume（4ml/kg）.At different time point before and after the experiment,we observed the changes of the oxygen pressure（PaO2）and oxygenation index（PaO2/FiO2）,wet/dry ratio,different expression of TREM-1and ICAM-1by immunohistochemical method,lung tissue pathological changes under the light microscope were observed.Result：The records in group A were basically normal.After injury in group B,PaO2and PaO2/FiO2decreased obviously,wet/dry ratio increased.The positive expression products on TREM-1and ICAM-1were increased aggregately.Under light microscope,alveolar septum became thicker and inflammatory cells infiltrated.The injury in group C was significantly lighter than that in group B.Conclusion：ARDS models of rabbits can be replicated with LPS intravenous injection（720μg/kg）.Both TREM-1and ICAM-1expressions were obviously detected in pulmonary tissues of ARDS animal models.Fufangkushen has certain protective effects on injury model animals.