鸟苷酸结合蛋白5和二磷酸腺苷核糖化因子鸟苷酸激酶1基因在肺结核患者和潜伏结核感染人群中的表达研究

Study on expression of the guanylate binding protein 5 and ArfGAP with SH3 domain ankyrin repeat and PH domain 1 genes among pulmonary tuberculosis patients and populations with latent tuberculosis infection

目的探讨鸟苷酸结合蛋白5（GBP5）和二磷酸腺苷核糖化因子鸟苷酸激酶1（ASAP1）基因在肺结核患者及潜伏结核感染人群中的表达情况。 方法选取2016年8月至2017年5月确诊肺结核患者40例（肺结核组）、潜伏结核感染患者40例（潜伏结核感染组）及健康对照者40例（健康对照组），取入选者外周抗凝血4 ml，应用实时荧光定量逆转录-聚合酶链反应（RT-PCR）技术检测这三组人群GBP5、ASAP1基因表达情况。 结果肺结核组、潜伏结核感染组和健康对照组GBP5基因相对表达量分别为1.58 ± 0.80、1.09 ± 0.68和1.04 ± 0.61，差异有统计学意义（F=7.23，P=0.001）；肺结核组GBP5基因相对表达量高于潜伏结核感染组（t=2.93，P=0.004）和健康对照组（t=3.40，P=0.010），差异有统计学意义；潜伏结核感染组GBP5基因相对表达量与健康对照组比较差异无统计学意义（t=0.39，P=0.700）。三组ASAP1基因表达量比较差异无统计学意义（F=0.26，P=0.770）。 结论GBP5基因表达在肺结核患者、潜伏结核感染患者和健康对照者中存在差异，GBP5基因检测可能筛选出潜伏结核感染人群，可作为筛选潜伏结核感染人群的标志物。

Objective To investigate the expression of guanylate binding protein 5 （GBPS） and ArfGAP with SH3 domain ankyrin repeat and PH domain 1 （ASAP1） genes in pulmonary tuberculosis patients and tuberculosis latent population. Methods Forty pulmonary tuberculosis patients （pulmonary tuberculosis group）, 40 latent tuberculosis infection patients （latent tuberculosis infection group） and 40 cases of healthy control （healthy control group） were selected from August 2016 to May 2017. The gene expression was detected in 4 ml peripheral anticoagulant blood by real-time fluorescent quantitative reverse transcription-polymerase chain reaction （RT-PCR） and the relative expression of two genes in three groups were compared. Results The GBP5 gene expression in three groups was significantly differentce （F=7.23, P=0.001）. The GBP5 gene relative expression in pulmonary tuberculosis group was significantly higher than that in latent infection group ：1.58 ± 0.80 vs. 1.09 ± 0.68, there was significant difference （t = 2.93, P = 0.004）. The GBP5 gene relative expression in pulmonary tuberculosis group was significantly higher than that in healthy control group： 1.58 ＋ 0.80 vs. 1.04 ± 0.61, there was significant difference （t= 3.40, P=0.010）. The GBP5 gene relative expression in latent infection group and healthy control group had no significant difference （t = 0.39, P = 0.700）. There was no significant difference in ASAP1 expression among three groups （F = 0.26, P = 0.770）. Conclusions The expression of GBP5 in pulmonary tuberculosis patients, latent tuberculosis infection patients and healthy controls is different, and GBP5 could screen latent tuberculosis infection patients which is expected to be a potential screening marker for latent tuberculosis infection.