Nf-κb在遗传性糖尿病长爪沙鼠多种组织中的表达状况

Expression Level of Nf-κb in Multiple Tissues from Hereditary Diabetic Mongolian gerbils

目的探讨糖尿病长爪沙鼠6种组织中Nf-κb基因mRNA和蛋白的表达水平,以期探索长爪沙鼠糖尿病模型中Nf-κb的作用和靶器官。方法选取糖尿病长爪沙鼠和正常血糖沙鼠各6只,分别采集动物的骨骼肌、肝脏、脂肪、肾脏、心脏和脑组织,用Q-PCR和Western blotting检测Nf-κb在各组织中mRNA和蛋白表达水平。结果与对照动物相比,在糖尿病长爪沙鼠的肌肉、肾脏和脑组织中,Nf-κb基因的mRNA水平表达有升高趋势,脂肪中则显著降低,肝脏和心脏中仅有降低趋势。检测蛋白水平发现除肝脏外,其他组织的结果均与mRNA水平一致。结论糖尿病长爪沙鼠脂肪组织是Nf-κb作用的靶器官。

Objective To investigate the mRNA and protein expression level of Nf-κb in 6 tissues of diabetic gerbils,and to explore the role and target tissue of Nf-κb in diabetic gerbil model. Method We selected 6 animals from diabetic and control group,respectively; and collected skeletal muscle,liver,adipose,kidney,heart and brain from each group. The expressionsat mRNA and protein level of Nf-κb were evaluated by Q-PCR and Western blotting in diabetic and control groups. Result Compared with control group,mRNA expression of Nf-κb had an increasing trend in skeletal muscles,kidney and brain of diabetic gerbil. The mRNA level of Nf-κb significantly decreased in adipose and showed decreasing tendency in liver and heart. After detecting protein expression,we found the protein level was consistent with mRNA in all tissues except liver. Conclusion Adipose tissue is the target tissue of Nf-κb in diabetic gerbil.