LncRNA CRNDE对脑胶质瘤细胞增殖、凋亡及迁移的影响

Effect of LncRNA CRNDE on the proliferation, apoptosis and migration of brain glioma cells

目的 探讨LncRNA CRNDE对脑胶质瘤细胞增殖、凋亡及迁移的影响.方法 将CRNDE基因干扰siRNA转染人胶质瘤U251细胞株,并同时设置空白组及对照组,利用PE AnnexinV-FITC染色,CCK-8实验以及细胞迁移实验分析LncRNA CRNDE对脑胶质瘤细胞凋亡、增殖及迁移的影响.结果 转染12h RNA干扰组（si783、si809）与对照组细胞480nm处吸光度的差异有统计学意义（P＜0.05）,与空白组之间差异无统计学意义（P＞0.05）;转染24 h及48 h干扰组（si783、si809）细胞480 nm处吸光度显著低于对照组和空白组（P＜0.05）;siRNA（si783、si809）的U251细胞凋亡比例分别为20.2％、19.3％,明显高于空白对照组（3.8％）及转染对照siRNA组（4.6％）（P＜0.05）,空白对照组与转染对照组之间差异无统计学意义（P＞0.05）.转染后干扰组（si783、si809）的细胞计数分别为（287.2±28.6）和（351.6±16.9）,均低于空白组（518.2±29.9）和对照组（471.6±29.4）（P＜0.05）,空白组与对照组之间差异无统计学意义（P＞0.05）.结论 CRNDE可促进胶质瘤细胞增殖及迁移,抑制胶质瘤细胞凋亡.

Objective To explore the effect of LncRNA CRNDE on the proliferation,apoptosis and migration of brain glioma cells.Methods LncRNA CRNDE was interfered by SiRNA in U251 ceLls.PE Annexin V-FITC staining and CCK-8 assay were employed for observing the effect of interfering LncRNA CRNDE on cell proliferation and apoptosis.And the capacity of cell migration was detected by scratch test.Results After 12 h transfection,statistically significant difference existed between control and SiRNA （si783,si809） groups.However,there was no statistically significant difference between SiRNA （si783,si809） and non-transfected groups.After 24 h,48 h transfection,statistically significant difference existed between SiRNA （si783,si809） group and control and nontransfected groups （P＜0.05）.The apoptosis rates were 20.2 ％ and 19.3 ％ in SiRNA （si783,si809）.Both were significantly higher than non-transfected group 3.8％ and control group 4.6％ （P＜0.05）.No significant differences existed between non-transfected group and control group （P＞0.05）.Cell migration experiment indicated that cell count of transfected group （287.2 ± 28.6） was lower than non-transfected group （518.2 ± 29.9） and control group （471.6 ± 29.4） （P＜0.05）.No significant differences existed between non-transfected and control groups （P＞0.05）.Conclusions CRNDE can effectively enhance cell proliferation and migration of glioma cells and decrease the rate of cell apoptosis.