电针对睡眠剥夺大鼠脾脏TLR4信号通路以及miR146a的影响

Electroacupuncture Has Effect on TLR4 Signal Pathway and miR 146a in Spleen of Rats with Sleep Deprivation

目的:观察电针对睡眠剥夺大鼠脾脏TLR4/NF-κβ信号通路上关键因子Toll样受体4(TLR4)、核因子-κβ(NF-κβ)、白细胞介素1受体相关激酶1(IRAK1)、肿瘤坏死因子受体相关因子6(TRAF6)和微小核糖核酸146a(miR146a)表达的影响。方法:将32只雄性SPF级Wistar大鼠随机分为对照组、模型组、安定组和电针组,每组8只。对照组未做任何处理,其余3组注射对氯苯丙氨酸(PCPA)进行睡眠剥夺后,安定组腹腔注射安定注射液,电针组给予电针百会、神庭,每组每天治疗1次,连续5天后采用RT-PCR检测大鼠脾脏TLR4、NF-κβ、IRAK1、TRAF6和miR146a的表达情况。结果:与对照组比较,模型组大鼠脾脏TLR4、NF-κβ、IRAK1、TRAF6的mRNA表达量明显升高,脾脏miR146a的表达量升高,差异均有统计学意义(P<0.05)。与模型组比较,安定组和电针组大鼠脾脏TLR4、NF-κβ、IRAK1、TRAF6的mRNA表达量显著降低,安定组大鼠脾脏miR146a的表达量显著降低,电针组大鼠脾脏miR146a的表达量明显升高,差异均有统计学意义(P<0.05)。结论:电针百会、神庭可以提高睡眠剥夺大鼠体内miR146a的表达,进而负性调节TLR4信号通路,最终达到对固有免疫应答的调控作用,减轻睡眠剥夺对机体的损害。

Objective：To observed the effect of electroacupuncture on the expression of key factors as Toll like receptor 4（TLR4）,Nuclear factorκβ（NF-κβ）,Interleukin 1 receptor related kinase1（IRAK1）,Tumor necrosis factor receptor related factor 6（TRAF6）,Micro RNA146a（miR146a）in TLR4/NF-κβsignal pathway in spleen of rats with sleep deprivation.Methods：Randomly divided 32male Wistar rats of SPF grade into the control group,model group,diazepam group and electroacupuncture group,8 rats in each group.The control group did not receive any treatment.After the other three groups received sleep deprivation by injection of para-chlorophenylalanine（PCPA）,the diazepam group received intraperitoneal injection of diazepam and the electroacupuncture group received electroacupuncture at Bai Hui and Shen Ting.Each group was given treatment once a day.After continuous treatment of five days,detected the expression of TLR4,NF-κβ,IRAK1,TRAF6 and miR146a in spleen of rats by RT-PCR.Results：Compared with the control group,the mRNA expression of TLR4,NF-κβ,IRAK1 and TRAF6 in spleen of rats was obviously increased and the expression of miR146a in spleen of rats was increased in the model group,differences being significant（P〈0.05）.Compared with the model group,the mRNA expression of TLR4,NF-κβ,IRAK1 and TRAF6 in spleen of rats in the diazepam group and the electroacupuncture group was obviously decreased,the expression of miR 146a in spleen of rats in the diazepam group was obviously decreased,and the expression of miR146a in spleen of rats in the electroacupuncture group was obviously increased,differences being significant（P〈0.05）.Conclusion：The therapy of electroacupuncture at Bai Hui and Shen Ting can improve the expression of miR146a in spleen of rats with sleep deprivation so as to negatively regulate TLR4 signal pathway,and eventually regulate innate immune response and relive the injury of body by sleep deprivation.