摘要
目的:对牛黄与其一种伪品进行鉴别研究。方法:将中药传统性状鉴定与体式显微和光学显微技术相结合,鉴定并报道了一种新发现的牛黄伪品。并应用高效液相色谱法及液质联用方法对染色物质进行了确证。结果:牛黄与该伪品在层纹的断面特征及颗粒状团块的显微特征等方面存在差异。该伪品主要是用淀粉和色素混合制作而成,伪品中掺有金胺O、皂黄、金橙Ⅱ、柠檬黄、胭脂红等5种染色物质。结论:从宏观到微观层级递进的观察方法可有效鉴别牛黄药材的真伪。HPLC-DAD和HPLC-MS等现代仪器分析方法可用于牛黄药材染色物质的确认。
Objective： To study the identification method of Bovis Calculus and one of its counterfeit. Methods： In this paper, a new kind of counterfeit of Bovis Calculus has been identified and reported, using the traditional macroscopic identification method combined with stereo and optical microscopy technology. And the method of HPLC and HPLC-MS were used to confirm the dyeing material. Result： There were some differences between Bovis Calculus and its counterfeit from the features of fracture striations and granulate mass observed under microscope. The counterfeit was mainly made by the mixture of starch and pigments. The results showed that the dyeing material was mixed with five kinds of pigments such as auramine O, metanil yellow, orange II, tartrazine and ponceau 4R. Conclusion： From macro to micro level progressive observation method is the necessary way to identify the authenticity of Bovis Calculus and its counterfeits. The application of modern instrumental analysis method such as HPLC-DAD and HPLC-MS is an important method to confirm the pigments of the counterfeit.
作者
康帅
林芳
穆向荣
连超杰
马双成
Kang Shuai;Lin Fang;Mu Xiangrong;Lian Chaojie;Ma Shuangcheng(National Institute for Food and Drug Control, Beijing 100050, China;Zhuzhou Institute for Food and Drug Control, Zhuzhou 412008, China;Shandong Institute for Food and Drug Control, Jinan 250101, China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2018年第3期405-412,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金(81173506):金银花咖啡酰奎宁酸类成分分离及其抗呼吸道合胞体病毒活性和作用机制研究
负责人:马双成
国家十二五"重大新药创制"专项课题(2014ZX09304307-002):中药质量安全检测和风险控制技术平台
负责人:马双成
关键词
真伪鉴别
牛黄
伪品
染色
方法
Authenticity identification
Bovis Calculus
counterfeit
pigments
identification
