奥尼罗非鱼肠道杯状细胞分布及分型观察

Distribution and Classification of Intestinal Goblet Cells in Tilapia Galilaea

本研究利用石蜡切片结合阿利新蓝-高碘酸Schiff(AB-PAS)和醛品红-阿利新蓝(AF-AB)染色法研究奥尼罗非鱼(Oreochromis niloticus♀×Oreochromis aureus♂)肠道内杯状细胞的分布与分型。结果发现,奥尼罗非鱼肠道黏膜层较厚,有大量肠绒毛突起,肠绒毛的数量从前肠到后肠逐渐减少。杯状细胞主要位于肠绒毛上皮细胞间,形状大多为椭圆或近似圆形,也有呈细长梭形。经统计分析可知,肠道黏膜上皮单位面积中杯状细胞数量从前肠到后肠逐渐增多,前肠、中肠、后肠杯状细胞平均数量分别为(230.0±4.9)个/mm^2、(287.9±7.5)和(336.7±10.0)个/mm^2,具有较大差异,三者间两两相比差异显著(P<0.05)。奥尼罗非鱼肠道杯状细胞以中性-酸性双染为主,酸性杯状细胞在肠道各段所占比例较低,且以硫酸化亚型杯状细胞为主,缺少羧酸化亚型杯状细胞。

The distribution and types of goblet cells in the intestine of Tilapia Galilaea（Oreochromis niloticus ♀ × Oreochromis aureus ♂） were studied by Alcian Blue-Periodic Acid Sthiff（AB-PAS） and Aldehyde Fuchsin-Alcian Blue（AF-AB） staining. The results showed that the intestinal mucosal layer of tilapia was thick, and a large number of intestinal villi decreased gradually from foregut to hindgut. Goblet cells were mainly located between the epithelial cells of intestinal villi, and their shape was mostly oval or approximately circular, and some were in a shape of a long and thin spindle（Fig. 1）. The statistical analysis showed that the number of goblet cells per unit area from foregut to hindgut gradually increased, the average numbers of goblet cells in foregut, midgut and hindgut of were 230.0 ± 4.9 cell/mm^2, 287.9 ± 7.5 cell/mm^2 and 336.7 ± 10.0 cell/mm^2, respectively, with significant difference among the three groups（P〈0.05, Table 1）. PAS and AB-PAS staining showed that the goblet cells in tilapia intestinal were mainly neutral and acid double staining, the acid goblet cells were low in the intestinal segments（Fig. 2, 3）, and the sulfuric acid cells were mainly sulfuric acid cells in AF-AB staining（Fig. 3）, and the cups of goblet cells were lack of carboxylic acid.