APOF和IGFALS基因在原发性肝癌组织中的表达及临床意义

Expression of APOF and IGFALS in primary hepatocellular carcinoma and its clinical significance

目的探讨载脂蛋白F(apolipoprotein F,APOF)和人胰岛素样生长因子酸不稳定亚基(the insulin-like grouth factor binding protein acid labile subunit,IGFALS)在原发性肝癌组织中的表达及临床意义。方法选取2014年1月1日至2015年12月30日于广西医科大学附属肿瘤医院经手术切除及病理确诊为原发性肝癌88例,实时荧光定量PCR(qRT-PCR)法检测88例肝癌组织及其相应癌旁组织和6例正常肝组织中APOF基因和IGFALS基因mRNA的相对表达量,并分析两者表达与肝癌患者临床病理特征的关系。结果肝癌组织中APOF mRNA相对表达量低于癌旁组织和正常肝组织(0.156±0.019 vs 0.971±0.010,P<0.001;0.156±0.019 vs 81.140±0.092,P<0.001);肝癌组织中IGFALS mRNA相对表达量亦低于癌旁组织及正常肝组织(0.111±0.016 vs 1.090±0.054,P<0.001;0.111±0.016 vs 1.101±0.211,P<0.001)。APOF mRNA表达与门静脉侵犯相关(P=0.004);IGFALS mRNA表达与肿瘤分化程度、门静脉侵犯相关(P<0.05)。APOF mRNA表达水平与IGFALS mRNA表达水平呈正相关(r=0.332,P<0.01)。结论 APOF和IGFALS基因在肝癌组织中表达下调,二者表达呈正相关,且与门静脉侵犯有关,可能参与了肝癌的发生发展。

Objective To investigate the expression and clinical significance of apolipoprotein F （APOF） and human insulin-like growth factor acid labile subunit （IGFALS） in primary hepatocellular carcinoma. Methods Levels of APOF and IGFALS mRNA were examined in 88 cases of human liver cancer and adjacent tissues and 6 cases of normal liver tissue using quantitative real-time PCR. Relationships between mRNA levels and clinico-pathological factors were analyzed. Results Levels of APOF mRNA were significantly lower in hepatocellular carcinoma tissues （0.156 ± 0.019） than in paracancerous tissue （0.971±0.018） or normal liver tissue （1.140±0.092）. Similarly, levels of IGFALS mRNA were significantly lower in hepatocellular carcinoma （ 0.111±0.016 ） than in adjacent tissues （ 1.090±0.054 ） or normal liver tissue （1.101±0.211, all P〈0.05 ）. Expression of APOF was associated with portal vein invasion （P=0.004）,while expression of IGFALS was associated with degree of differentiation and portal vein invasion （P〈0.05）. Expression of APOF and IGFALS was lower among patients showing portal vein invasion than among those without portal vein invasion （P〈0.05）. Expression of IGFALS was lower in the low- differentiation group than in the high-differentiation group（P〈0.05）. Conclusions APOF and IGFALS are down-regulated in hepatocellular carcinoma tissues. Expression of the two genes positively correlates with each other, and their expression correlates with the extent of portal vein invasion and differentiation. These genes may be involved in the occurrence and development of hepatocellular carcinoma.