摘要
目的:比较电针、二甲双胍、电针配合二甲双胍治疗对多囊卵巢综合征(PCOS)模型大鼠的作用。方法:40只大鼠随机分成正常对照(D)、模型(M)、电针(A)、二甲(B)组、与二甲针(C)等5组,以丙酸睾丸酮合并高脂饲料造模;分别给予电针(A、C组)和/或二甲双胍(B、C组)治疗,检测各项指标。结果:A、B、C组的睾酮(T)、肿瘤坏死因子-α(TNF-α)、空腹胰岛素(FINS)、和胰岛素抵抗指数(HOMA-IR)显著降低;部分大鼠出现规律的动情周期,黄体数目及卵泡内颗粒细胞层数增加,囊性卵泡减少。结论:A、B、C方案对PCOS大鼠均有疗效。电针结合二甲双胍对糖耐量的调节较单纯电针更快显效。
Objective : To compare the effects of eletro - acupuncture, metformin, and eletro - acupuncture combined with metformin on the polycystic ovary syndrome (PCOS) rats. Methods: Forty SD rats were randomly designated into five groups, i.e. normal (D) group, model (M) group, eletro - acupuncture (A) group, metformin (B) group, and eletro -acupuncture combined with mefformin (C) group. PCOS was induced in 3 -week -old rats with testosterone propio- nate (TP) subcutaneous injection (10 mg/kg per day for consecutive $ weeks) and high -fat diet feeding (HFD, main- tained to the end of the experiment). Animals in A and C groups were given electro - acupuncture (2 Hz for 30 minutes per day, five days per week for consecutive five weeks) , while animals in B and C groups were given metformin (250 mg/kg per day for consecutive five weeks). Blood testosterone(T), TNF - α, fasting insulin ( FINS), fasting blood glu- cose ( FBG), HOMA - IR, and oral glucose tolerance test (OGTI') were tested or measured before and after treatment. Results: The blood testosterone, TNF -α, FINS, HOMA -IR, and OGTT (30 -, 60 - min) in the A, B and C groups were lower than that in the M group (P 〈 0. 05 ), while there was no significant difference in the 120 min OGTT between the A group and the M group. Compared with the M group, the A, B and C groups showed growing follicles and some corpora lutea ovaries. And some rats in A, B and C groups showed normal estrum after intervention. Conclusion: Treat- ments of A, B, and C groups lead to declined TNF-α expression, ameliorated insulin sensitivity, controlled body weight, down - regulated testosterone, and partially recovered estrous cycle. Metformin or metformin combined with elec- tro -acupuncture were better than electro -acupuncture in regulating 120 min OGTT.
作者
孟延兵
郑艳华
王素玲
马红霞
MENG Yanbing;ZHENG Yanhua;WANG Suling;MA Hongxia(The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou 510700, Guangdong, China;The Fourth Affiliated Hospital of Guangzhou Medical University, Guangzhou 511447,Guangdong,China;The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120,Guangdong,China)
出处
《中华中医药学刊》
CAS
北大核心
2018年第6期1376-1379,I0025,共5页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81574013)
关键词
多囊卵巢综合征
电针
二甲双胍
胰岛素抵抗
polycystic ovary syndrome
electro - acupuncture
metformin
insulin resistance