HPLC测定加味升降散中5种大黄蒽醌类成分含量

Determination of Five Rhubarb Anthraquinones Constituents in Shengjiang Powder by HPLC

目的：建立加味升降散中5种大黄蒽醌类成分（芦荟大黄素、大黄酸、大黄酚、大黄素、大黄素甲醚）含量的测定方法。方法：YMC-Pack ODS-AM C18色谱柱（250 mm×4.6 mm,5μm）,柱温：25℃;以甲醇为流动相A,0.1%磷酸溶液为流动相B,梯度洗脱,检测波长254 nm,流速1.0 m L·min-1。结果：芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.257 42.059 0,0.163 51.308 2,0.098 20.785 7,0.415 13.321 0,0.080 40.643 1μg范围内线性关系良好（r≥0.999 8）,平均加样回收率（n=9）分别为97.01%（RSD 1.28%）,99.67%（RSD 2.17%）,98.97%（RSD 2.3%）,99.52%（RSD 1.95%）,99.04%（RSD 1.82%）。结论：实验中建立的研究方法重现性好、操作简便、准确可靠,可应用于加味升降散水煎剂的质量控制。

Objective： To establish the determination method of the five Rhubarb anthraquinones constituents in Shengjiang Powder which contained the aloe - emodin, rhein, chrysophanol, emodin and rheochrysidin. Methods ： The a- nalysis was achieved on a YMC -Pack ODS -AM Cls column （250 mm×4.6 mm,5 μm） with the mobile phase of meth- anol（A） -0.1% phosphate acid（B） ,at a flow rate of 1.0 mL · min-1. The detection was at 254 nm and temperature was at 25 ~C. Results ： The liner rangers of aloe - emodin, rhein, emodin, chrysophanol and rheochrysidin were within 0. 257 4 - 2.059 0 μg（ r = 0.989 9 ） ,0. 163 5 - 1. 308 2 μg（ r = 0. 998 ） ,0. 098 2 - 0.785 7 μg（ r = 0. 999） ,0.415 1 - 3. 321 0 μg（ r = 0. 999 9 ） , 0. 080 4 - 0. 643 1 μg （ r = 0. 999 8 ）. The average recovery rates were 97.01% （ RSD 1.28% ） ,99.67% （ RSD 2.17% ） ,98.97% （ RSD 2.33% ） ,99.52% （ RSD 1.95% ） ,99.04% （ RSD 1.82% ）, respec- tively（ n = 9）. Conclusion： This method is easy,reproducible, accurate and reliable,which could be used for quality con- trol of Shengiiang Powder.