基于结肠黏膜JAK2/STAT3通路研究健脾化痰法的作用机制

Effect of Xiangsha Liujun Pill on Colonic Mucosal JAK2/STAT3 Pathway in Spleen Deficiency and Phlegm Turbid Rats

目的：通过观察香砂六君丸对脾虚痰浊大鼠结肠黏膜JAK2/STAT3通路的影响,探讨健脾化痰法的作用机制。方法：40只雄性SD大鼠,随机分为对照组、脾虚痰浊组（模型组）、香砂六君丸低剂量治疗组（香低组）、香砂六君丸中剂量治疗组（香中组）和香砂六君丸高剂量治疗组（香高组）,每组8只;全自动生化分析仪检测血清总甘油三酯（TG）;ELISA法检测血清超氧化物岐化酶（SOD）、丙二醛（MDA）和脂多糖（LPS）水平及谷胱甘肽过氧化物酶（GSH-Px）活性;免疫组化法检测结肠黏膜磷酸化（p）-JAK2、p-STAT3和Bcl-2表达。结果：与对照组比较,模型组SOD水平、GSH-Px活性和Bcl-2表达均下降,MDA、LPS水平和p-JAK2/STAT3表达升高,均有统计学意义;与模型组比较,后3组SOD水平、GSH-Px活性和Bcl-2表达上升,后2组的MDA水平减少,后3组的LPS水平下降,后3组p-JAK2表达减少,均有统计学差异;与香低组比较,后2组MDA浓度下降,均有统计学意义。结论：健脾化痰法能够对抗氧化应激,抑制内毒素过度释放,干预结肠黏膜细胞JAK2/STAT3通路以增强其抗凋亡能力。

Objective： To study the effect of Xiangsha Liujun Pill（XLP） on colonic mucosal JAK2/STAT3 pathway in spleen deficiency and phlegm turbid rats, attempting to explore the action mechanism of the principle of invigorating spleen. Methods： Forty male SD rats were randomly divided into the control group, spleen deficiency and phlegm turbid group （model group）, XLP low- dose treating group （XLG）, XLP middle -dose treating group （XMG） and XLP high - dose treating group （XHG）, 8 rats in each group. Automatic chemistry analyzer was used to measure serum total glyc- eride level. ELISA was employed to detect serum levels of superoxide dismutase （SOD）, malondialdehyde （MDA） and lipopolysaccharide （LPS） and glutathion peroxidase （GSH -Px） aetiviability. The immunohistochemistry was used to detect expressions of phosphorylated （p） -JAK2, p- STAT3 and Bcl -2 in colon muscosa. Results： Compared with those in the control group, SOD level, GSH - Px activitablity and Bcl - 2 expression were decreased, while MDA and LPS levels and p -JAK2/STAT3 expressions were increased significantly in the model groups. Compared with those in the model group, SOD level, GHS -Px aetivitability and Bcl -2 expression in later three groups were increased signifi- cantly, while MDA levels in later two groups and LPS levels and p -JAK2 expression in later three groups were lowered obviously. Compared with that in XLG, MDA levels in later two groups were decreased significantly. Conclusion： The principle of invigorating spleen and resolving phlegm can opposite oxidative stress, inhibit endotoxin overrelease and improve the anti - apoptotic ability of the colonal mueosa via interpreting JAK2/STAT3 pathway.