养胃解毒合剂对脓毒症大鼠肠组织凋亡因子Bax、Bcl2和Caspase3的影响

Effects of Yangwei Jiedu Mixture on Intestinal Tissue of Rats with Sepsis Apoptosis Factors Bax,Bcl2 and Caspase3

目的：通过观察养胃解毒合剂对脓毒症大鼠肠组织TNF-α的表达及Bax、Bcl2、Caspase3相关凋亡蛋白的影响,探讨养胃解毒合剂对脓毒症大鼠的肠保护机制。方法：140只大鼠分为7组,正常组20只、模型组20只、中药正常剂量组20只、中药高剂量（2倍）组20只、西药组（泰能）20只、西药＋中药正常剂量组20只、西药＋中药高剂量组20只。通过HE染色观察肠组织病理改变;ELISA法对TNF-α的表达进行检测;采用Western Blot（WB）法检测Bax、Bcl2、Caspase3蛋白表达。结果：与正常组比较,模型组TNF-α含量明显升高,Bax、Caspase3蛋白表达上调,Bcl2蛋白表达下调（P〈0.01）,西药＋中药高剂量联合治疗后TNF-α表达明显减少（P〈0.01）。西药＋中药高剂量组肠组织Bax和Caspase3蛋白表达水平下调,Bcl-2蛋白表达水平上调（P〈0.01）。结论：养胃解毒合剂可能通过抑制TNF-α的表达,介导调控肠组织的Bax、Bcl2、Caspase3凋亡蛋白,起到对脓毒症大鼠的肠保护作用。

Objective： To demonstrate the mechanism of Yangwei Jiedu Mixture in rescuing intestine of sepsis rats by investigating TNF - α, Bax, Bcl2 and Caspase3 protein expressions. Method： A total of 140 SD rats were randomly divid- ed into 7 groups （20 rats in each） as following ： control group, model group, Yangwei Jiedu Mixture regular dose group, Yangwei Jiedu Mixture high dose group, TIENAM group, Yangwei Jiedu Mixture regular dose plus TIENAM group and Yangwei Jiedu Mixture high dose plus TIENAM group. Histology was used to observe the pathogenic change in intestine tissue. ELISA was used to compare TNF - α expression in intestine. Western blotting was used to examine the protein ex- pressions of Bax, Bcl2 and Caspase3. Result ： Comparing to control group, model group showed higher protein expressions of TNF - α,Bax and Caspase3 and lower expression of Bcl2 significantly（P 〈0. 01 ）. And TNF - α, Bax and Caspase3 were significantly down - regulated （ P 〈 0. 01 ） and Bcl - 2 was dramatically up - regulated（ P 〈 0. 01 ） in Yangwei Jiedu Mixture high dose plus TIENAM group. Conclusion： Yangwei Jiedu Mixture can protect intestine of sepsis rats by inhibi- ting TNF-α expression to regulate apoptosis - relevant protein Bax, Bcl2 and Caspase3.