DNMT1基因rs16999593位点的多态性与乳腺癌易感性关系的meta分析

Association between polymorphisms of DNMT1 rs16999593 and susceptibility of breast cancer: a meta-analysis

目的采用meta分析的方法评价DNA甲基转移酶1(DNA methyltransferase 1,DNMT1)基因rs16999593位点的多态性与乳腺癌发病的关系。方法计算机检索PubMed、EMbase、Web of Science、中国知网、万方、中国生物医学文献数据库及维普数据库中关于DNMT1基因多态性与乳腺癌发病关系的病例对照研究,检索时间均为建库至2017年3月。由两位评价者按照纳入和排除标准独立筛选文献,并对纳入文献进行方法学质量评价。提取相关资料,采用RevMan 5.3软件进行meta分析。结果最终纳入5项病例对照研究,共计1 741例乳腺癌患者和1 917位健康对照者。meta分析结果显示:显性遗传模型[OR=0.63,95%CI为(0.30,1.30),P=0.21]、纯合子模型[OR=1.01,95%CI为(0.70,1.47),P=0.95]、杂合子模型[OR=0.44,95%CI为(0.18,1.04),P=0.06]及等位基因模型[OR=1.29,95%CI为(0.90,1.86),P=0.16]均与乳腺癌的发生无关,但隐性遗传模型[OR=1.74,95%CI为(1.01,3.00),P=0.04]与乳腺癌的发生有关。结论在亚洲人群中,DNMT1基因rs16999593位点的TT基因型是乳腺癌发病的保护因素。

Objective To systematically review the correlation between polymorphism of DNA methyltransferase 1（DNMT1） rs16999593 and the susceptibility of breast cancer. Methods Databases such as PubMed, EMbase, Web of Science, Chinese Biomedical Literature Database, CNKI, WanFang, and VIP database were searched from inception to Mar. 2017 to collect case-control studies on the correlation between DNMT1 rs16999593 C/T polymorphism and the susceptibility of breast cancer. Two reviewers independently identified the literatures according to inclusion and exclusion criterias, extracted data, and assessed the quality of the included studies. The meta-analysis was performed by using RevMan 5.3 software. Results A total of 5 studies involving 1 741 cases and 1 917 control subjects were included. The results of meta-analysis showed that, dominate model [TT＋TC vs. CC： OR=0.63, 95% CI was （0.30, 1.30）, P=0.21], homozygous model [TT vs. CC： OR=1.01, 95% CI was （0.70, 1.47）, P=0.95], heterozygous model [TC vs. CC： OR=0.44, 95% CI was （0.18, 1.04）, P=0.06], and additive model [T vs. C： OR=1.29, 95% CI was （0.90, 1.86）, P=0.16] were not significantly related to breast cancer, but recessive gene model was related to breast cancer [TT vs. TC＋CC： OR=1.74, 95% CI was （1.01, 3.00）, P=0.04]. Conclusion The current studies showed that, DNMT1 rs16999593 TT genotype decreases the susceptibility of breast cancer.