靖安白茶芽和叶的转录组数据组装及基因功能注释

Transcriptome Data assembly and Gene Function Annotation of Buds and Leaves of Camellia sinensis Cultivar Jing'anbaicha

茶树富含茶多酚,茶氨酸等多种具有广泛保健功能的植物代谢产物,黄酮是一类主要的茶多酚成分,谷氨酰胺是合成茶氨酸的前体物质。本研究拟从转录组水平阐明靖安白茶茶多酚,茶氨酸等产物代谢途径相关基因。本研究主要以靖安白茶芽和老叶为材料,采用Illumina高通量测序平台4000(Hi Seq/Mi Seq 4000)对其芽和老叶进行转录组测序,通过多种生物信息学方法对转录组数据进行分析。测序结果共得到100568条unigene序列,碱基数为95121064 bp,Nr获得32530个功能注释,KOG获得30087个功能注释,GO获得29010个功能注释,Swiss-Prot获得24553个功能注释,Pfam获得18576个功能注释,并通过GO富集分析和KEGG代谢通路富集分析发掘靖安白茶芽和叶中参与黄酮类化合物和谷胱甘肽化合物代谢途径相关的差异unigene序列。通过本研究为进一步深入的研究靖安白茶的茶多酚和茶氨酸等代谢产物生物合成调控提供依据。

Plant metabolites such as tea polyphenols, theanine, with extensive health benefits, are extremely rich in tea tree. Flavonoids are the main component of tea polyphenols, while glutamine is the precursor substance of the synthesis of theanine. This study attempted to elucidate the related genes of the metabolisms of tea polyphenols and theanine of Jing＇an white tea. Fresh buds and leaves of Camellia sinensis cultivar Jing＇anbaicha were selected as samples, the transcriptome was constructed using Illumina Hi Seq TM 4000 sequencing technique and the data were analyzed using the bioinformatics methods subsequently. 100568 unigene sequences were obtained with length of 95121064 bp. Gene function annotations analysis showed that 32530 annotations were acquired from Nr database, 30087 annotations were acquired from KOG database, 29010 annotations were acquired from GO database, 24553 annotations were acquired from Swiss-Prot database, 18576 annotations were acquired from Pfam database, respectively. The key differences unigene sequences related to the metabolic pathway of flavonoids and glutathione in Jing＇an white tea buds and leaves were explored through the GO analysis and the KEGG metabolic pathway analysis. This study could provide references for further study of the biosynthesis regulation of tea polyphenols and theanine in Jing＇an white tea.