PeriCam PSI血流灌注成像系统在脑缺血再灌注动物模型中的应用及评价

Application of Peri Cam PSI blood flow imaging perfusion system in rat brain ischemia reperfusion injury models

目的:研究Peri Cam PSI血流灌注成像系统指导大鼠脑缺血再灌注损伤模型构建的可行性和效果。方法:成年Sprague-Dawley雄性大鼠70只随机分为手术对照组(n=6)、PSI监测组(n=34)和传统方法组(n=30)。参照改良ZeaLonga栓线法制备大鼠大脑中动脉缺血再灌注模型,建模后,手术对照组和PSI监测组(缺血2 h、再灌注24 h)分别用PSI对脑部血流进行实时监测,记录观察结果。恢复再灌24 h后处死大鼠,取脑组织进行2,3,5-三苯基氯化四氮唑(TTC)染色和HE染色。结果:PSI监测组大鼠的存活率和建模成功率均高于传统方法组(均P<0.05)。手术对照组大鼠脑血流灌注图像可以清晰地观察到脑部血流灌注情况和血管分布,各项数据正常。PSI监测组缺血2 h,右侧大脑中动脉血流出现中断,中动脉供血区血流灌注量较手术对照组下降(P<0.05);恢复再灌注24 h,右侧大脑中动脉恢复血流灌注,但是仍然有部分区域的血流灌注量下降,无法恢复到正常水平。TTC染色结果显示,PSI监测组大鼠右侧脑组织存在明显的梗死灶,且脑梗死区域较传统方法组稳定。HE病理染色结果显示,手术对照组脑组织结构正常,神经细胞形态规则,无明显异常;PSI监测组大鼠缺血侧脑组织可见皮质区和缺血半暗带区,神经细胞变性、坏死、胶质纤维崩解、液化,缺血中心区淡染、呈筛网状,部分区域呈灶性坏死。结论:Peri Cam PSI血流灌注成像系统可以指导缺血再灌注模型的构建,提高建模的成功率和稳定性。

Objective： To study the feasibility and effect of Peri Cam PSI system guiding the establishment of ischemia/reperfusion injury model in rats. Methods： A total of 70 adult male Sprague-Dawley rats were divided into the control group（ n = 6）,PSI monitoring group（ n = 34） and traditional operation group（ n = 30）. Ischemia reperfusion model was established with reference to improve Zea-Longa line plug method. After the model established,the blood flow to the brain of control group,PSI monitoring group（ ischemic 2 h,24 h reperfusion） were observed and recorded respectively with PSI. The rats were then executed after 24 h, and the 2,3,5-triphenyltetrazolium chloride（ TTC） staining and HE staining were used to observe the brain tissue. Results： The survival rate and modeling success rate of PSI monitoring group were higher than those of the traditional operation group（ all P〈0. 05）. The blood perfusion in the brain and the distribution of blood vessels were clearly observed in the control group,and the data were normal. In 2 h ischemic group,the arterial flow was interrupted in the right cerebral artery,and the blood flow in the middle arterial blood supply was significantly decreased than that in the control group（ P〈0. 05）.After the recovery of 24 h,the artery in the right side of the brain was restored to blood flow,but the blood flow in the partial supply area decreased,unable to recover to normal level. The TTC staining results indicated that there were obvious infarcts in the right brain tissue of PSI monitoring group,and the infarct area was more stable than that of the traditional operation group. The results of HE staining showed that the structure of brain tissue in the control group was normal,and the morphological rules of nerve cells were not change. While in brain tissue from PSI monitoring group,cortex and ischemia half dark stripe, nerve cell degeneration, necrosis and glial fiber disintegration,liquefaction,and light color,screen mesh in ischemic central area were observed. Conclusion： PSI system can guide ischemia reperfusion model building and improve the success rate of the model.