摘要
CRISPR-Cas system has been widely adapted as a platform for genome editing in various eukaryotic organisms, including zebrafish(Cong et al., 2013; Hwang et al., 2013). One of the important applications of CRISPR-Cas9 system is to produce double-strand DNA breaks(DSBs) at targeted sites with guide RNA(gRNA).
CRISPR-Cas system has been widely adapted as a platform for genome editing in various eukaryotic organisms, including zebrafish(Cong et al., 2013; Hwang et al., 2013). One of the important applications of CRISPR-Cas9 system is to produce double-strand DNA breaks(DSBs) at targeted sites with guide RNA(gRNA).
基金
supported by the funding from the Science and Technology Program of Shenzhen (JCYJ20150924110425180 and JCYJ20151030170755264)