酚妥拉明加林可霉素利多卡因凝胶处理胺碘酮输注外渗致静脉炎的动物实验研究

Treatment of Phentolamine combined with Lincomycin Hydrochloride and Lidocaine Hydrochloride Gel for phlebitis caused by amiodarone extravasation： an animal study

目的评价酚妥拉明加林可霉素利多卡因凝胶在处理胺碘酮输注外渗在动物实验中效果。方法2017年3月选择成年兔15只，进行胺碘酮损伤模型建立。按照体重从轻至重排序编号，采用随机数字表法分为5组，A为模型组，B为50％硫酸镁外敷组，C为酚妥拉明外敷组，D为林可霉素利多卡因凝胶外敷组，E为酚妥拉明加林可霉素利多卡因凝胶外敷组，比较5组之间以及其他4组与A组在肉眼观察、病理观察、免疫组化方面的差异。结果胺碘酮输液外渗5组不同处理后在第1天症状观察疗效差异无统计学意义（P〉0．05），在治疗后第2、3天，5组间治疗效果差异有统计学意义（P〈0．01），两两比较发现在第2天D组与A组、E组和A组比较差异均有统计学意义（P〈0．05），在外渗后处理的第3天D组与A组、E组与A组比较差异均有统计学意义（P〈0．05）。胺碘酮外渗5组不同方式处理3d后兔耳静脉及周围组织病理损伤程度比较差异均有统计学意义（P〈0．05）。5组VEGF平均阳性率差异有统计学意义（P〈0．001），A组平均阳性率明显高于其他4组，E组低于其他4组，差异均有统计学意义（P〈0．05），B、C和D组比较差异无统计学意义（P〉0．05）。结论酚妥拉明加林可霉素利多卡因凝胶在处理胺碘酮输注外渗的动物实验有明显效果。

Objective To evaluate the effects of Phentolamine combined with Lincomycin Hydrochloride and Lidocaine Hydrochloride Gel for phlebitis caused by amiodarone extravasation in an animal study. Methods A total of 15 adult rabbits were selected in March 2017. We built a model according to amiodarone damage. All of the rabbits were numbered by weight from light to heavy and divided into five groups with the random number table including group A （model group）, group B （group with 50% magnesium sulfate for external application）, group C （with Phentolamine for external application）, group D （with Lincomycin Hydrochloride and Lidocaine Hydrochloride Gel for external application） and group E （Phentolamine combined with Lincomycin Hydrochloride and Lidocaine Hydrochloride Gel for external application）. We compared the differences in naked eye observation, pathological observation and immunohistochemistry among five groups and compared the differences of group A with other four groups. Results There were no significant differences in effects of symptom observation among five groups on the first day after different treatments for amiodarone extravasation （P 〉 0.05）. The differences in treatment effects were significant among five groups on the second and third day after treatments （P 〈 0.01）. There were significant differences between group D and group A, group E and group A on the second and third day after treatments for extravasation （P 〈 0.05）. The differences in ear vein and surrounding tissues pathological injury of rabbits were significant among five groups on the third day after different treatments for amiodarone extravasation （P 〈 0.05）. There were significant differences in average positive rates of vascular endothelial growth factor （VEGF） among five groups （P 〈 0.001）, the average positive rate of VEGF in group A was higher than that in other four groups （P 〈 0.05） ; the average positive rate of VEGF in group E was lower than that in other four groups with significant differences （P 〈 0.05） ; there was no significant differences among group B, group C and group D （P 〉 0.05）. Conclusions The Phentolamine combined with Lincomycin Hydrochloride and Lidocaine Hydrochloride Gel for phlebitis caused by amiodarone extravasation has obvious effects in an animal study.