以类平均聚类法分析不同产地土贝母中土贝母苷甲含量及影响因素

Content and influencing factors of tobeimoside Ⅰ in Tubeimu from different producing areas analyzed by using group-average clustering method

目的测定16批不同产地土贝母中土贝母苷甲含量,确定土贝母含量高的产区,分析影响含量的因素。方法采用《中国药典》2015版土贝母药材含量方法测定土贝母苷甲;以土贝母苷甲含量为指标用类平均聚类分析法将产地来源进行分类。结果不同产地土贝母中苷甲含量在1.234%~3.174%范围内,符合药典要求;聚类分析可将16个产地分为4类,来自商洛、汉中的一类药材苷甲含量最高。结论温度和湿度可影响土贝母药材质量。温度、湿度偏高地区的土贝母药材中土贝母苷甲含量更高。

Objective To identify the content of tubeimoside Ⅰ in Paniculate Bolbostemma from 16 different locations and determine the high-containing locations and influencing factors. Methods The quantitative determination method of tubeimoside Ⅰ in Tubeimu（Paniculate Bolbostemma,Rhizoma Bolbostematis） in Chinese Pharmacopoeia（2015 Edition） was used to determine the content of tubeimoside Ⅰ in 16 batches of Tubeimu for deciding producing areas with higher content of Tubeimu and analyzing the influencing factors. The origin producing areas was classified by using group-average clustering analysis method taken content of tubeimoside Ⅰ as the index. Results The results showed that the content of tubeimoside Ⅰ was in a range from 1. 234% to 3. 174% in different producing areas,which was accorded with the requirement of Chinese Pharmacopoeia. The clustering analysis classified 16 producing areas into 4 groups,and content of tubeimoside Ⅰ was the highest in Tubeimu material produced from Shangluo and Hanzhong. Conclusion The temperature and humidity had influence on Tubeimu material,and content of tubeimoside Ⅰ was higher in the producing areas with higher temperature and humidity.