青梅酵素的生物活性及体外抑菌作用

Bioactivity and inhibiton effects of greengage ferment

对青梅酵素的抗氧化活性、酶活力以及抑菌作用进行了研究。结果表明,青梅酵素的抗氧化活性和酶活力较强。体积浓度为0.25%时,总抗氧化性与100μg/m L VC的抗氧化性相当;体积浓度为45%时,·OH清除率为83.2%;体积浓度为8%时,对DPPH·清除率达到96.5%;体积浓度为15%的青梅酵素还原力相当于浓度为100μg/m L的VC溶液。体积浓度为30%时,超氧化物歧化酶(SOD)活力最高,为75.64 U/m L;浓度为15%时,蛋白酶活力为15.84 U/m L;脂肪酶活力较低,为1.67 U/m L。青梅酵素原液对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的抑制作用较强,抑菌圈直径分别为(19.9±0.56)、(17.2±0.15)、(19.0±0.3)mm。

In this paper the antioxidant activity and enzyme activity and bacteriostasis of greengage ferment were studied. The results showed that the antioxidant activity and enzyme activity of greengage ferment were strong.Total oxidation resistance was equal to oxidation resistance of 100 μg/m L Vit C when greengage ferment volume concentration was 0.25%. When greengage ferment＇s volume concentration was 45%,OH·clearance rate reached 83.2% and DPPH·clearance rate reached 96.5% when the volume concentration was 8%.The reducing power of greengage ferment was equal to reducing power of 100 μg/m L Vit C when greengage ferment volume concentration was 15%.When greengage ferment＇s volume concentration was 30%,greengage enzyme superoxide dismutase（ SOD） had the highest activity,which was 75.64 U/m L. When greengage ferment ＇s volume concentration was 15%,protease activity was 15.84 U/m L and lipase activity was lower,which was 1.67 U/m L. Inhibition effect on Staphylococcus aureus,Escherichia coli,Bacillus subtilis inhibitory was strong.The inhibition zone of Staphylococcus aureus,Escherichia coli,Bacillus subtilis respectively were（ 19.9 ± 0.56）,（ 17.2 ± 0.15）,（ 19.0 ± 0.3） mm.